Product Focus: Anti-mouse CD31 / DIA-310 Rat monoclonal anti-mouse endothelial cell marker CD31 (PECAM-1), Clone SZ31

Antibody clone SZ31 is the first antibody which reacts specifically with murine CD31 in formalin-fixed paraffin-embedded tissue sections.

CD31, also known as PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1) is expressed constitutively on the surface of embryonic and adult endothelial cells. It is also expressed on cell surfaces of monocytes, neutrophils, platelets and certain T-cell
subsets. It has been detected on bone marrow-derived hematopoetic stem cells and embryonic stem cells. CD31 is a 130kDa integral membrane glycoprotein and as a member of the immunoglobulin superfamily involved in the mediation of cell-to-cell adhesion.

CD31-mediated endothelial cell-cell interactions play a major role in angiogenesis. Studies have shown CD31 to be a superior marker in human angiogenesis, which reportedly predicts tumor recurrence. Pathophysiological studies of CD31 in murine model systems had limitations because standard formalin-fixed sections were excluded. The clone SZ31 eliminates these restrictions by allowing high quality immunohistochemical analysis of standard formalin-fixed paraffin sections in mice.


Product Information

MSDS        Datasheet

Catalogue numbers: DIA-310 (100μg)
DIA-310-M (20μg)
Specificity: CD31
Species Reactivity: Mouse
Host Species: Rat
Isotype: IgG2a
Clone: SZ31
Clonality (Mono-/Polyclonal): monoclonal
Application: Immunohistochemistry (IHC), Paraffin Sections (PS), Frozen Sections (GS), Western Blot
Conjugation: unconjugated
Format: lyophilisate, antibody purified (from culture supernatant)
Intended Use: for Research Use Only
Research Area: angiogenesis, CD markers, immunohistochemistry

Where can I find more information about Dianova?

Visit the manufacturer page at www.stratech.co.uk/dianova, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


 

Product Focus: HR Targeting Vectors for CRISPR/Cas9 and TALE-based Editing

CRISPR/Cas9 and TALE-Nuclease technologies have provided researchers the ability to edit any genomic locus with site-specific DNA cutting. In order to fully leverage the power of these genome engineering nucleases, one can use homology-directed recombination for targeted insertions at DNA cleavage sites. For Knock-Out applications, using HR donor vectors enables positive selection of engineered cells, greatly reducing the amount of screening needed to isolate bi-allelic KO clones. For Gene Editing and Tagging applications, an HR template is required to introduce the desired nucleotide modifications or reporter tags. SBI has generated a suite of homologous recombination donor vectors to improve the efficiency of any genome engineering application.

Features include:

  • Simple HR arm additions flanking insulated expression cassettes
  • Built-in LoxP sites for downstream cassette removal
  • Detailed Application Notes providing step-by-step protocols
  • Live phone support from experienced genome engineering specialists
  • Custom HR Donor Design & Construction Services

How do I Knock-out and Knock-in genes using HR vectors?

For HR-based applications needing the disruption of a particular protein-coding or non-coding gene of interest, a gene knock-out targeting vector can be used. The HR process is initiated by site-specific DNA cleavage using either a CRISPR/Cas9 or TALE-Nuclease vector set within the region of interest. Next, simply clone 5′ and 3′ genomic sequences immediately flanking the targeted DNA sequence of interest to specifically direct the HR vector to insert an expression cassette into the targeted genomic location. This leads to the ‘knock-out’ of the sequence by HR cassette addition. The HR cassettes included in the PrecisionX vectors feature RFP, GFP and/or Puromycin/Hygromycin to facilitate the identification of cells that have undergone accurate genome engineering.

SBI Application Note for CRISPR-Cas9_Gene Knock-Out


How can I introduce single base changes into a target gene using HR vectors?

Learn how to introduce single base modifications into target genes for gene corrections and mutations. SBI provides HR vectors, CRISPR/Cas9 vectors and TALE-Nuclease systems to accomplish finely-targeted gene editing applications. Become a gene surgeon and create unique model cell lines with this powerful set of technologies.

SBI Application Note for CRISPR-Cas9 SBP Gene Editing


How can I tag endogenous genes for tracking using HR vectors?

Tag an endogenous sequence of interest with a marker to create a fusion or to co-express a marker tag with the endogenous sequence for various applications (e.g. tracking spatial dynamics of a protein, lineage tracking, etc.) with HR-tagging vectors. SBI provides a collection of four tagging and co-expression vectors for HR-based applications. Any type of DNA sequence, from protein-coding ORFs to non-coding sequences can be efficiently tagged or co-expressed with GFP and/or luciferase using these vectors. For protein-coding genes, the 3′ end (typically the last exon) can be targeted using these vectors for efficient generation of C-terminal fusion tagged proteins or co-expression of the gene and the marker tag via T2A or IRES elements.


Resources

Technical References


Where can I find more information about SBI?

Visit the manufacturer page at www.stratech.co.uk/SBI, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


 

Product Focus: Lyophilized PCR mixes – No fridge required!

No freezing or cooling during storage, shipping and handling!

Jena Bisocience’s Freeze-dried (lyophilized) PCR mixes are stable at ambient temperatures up to 30 °C and allow point-of-care usage without any refrigerating infrastructure.

Vials containing the freeze-dried pellet are simply filled up with template DNA, primers and PCR-grade water, and the PCR can be run as usual. Lyophilized PCR mixes are available in single PCR tubes, 8-well tube strips, 96-well plates or custom-specific formats. In addition, we offer adaption to individual applications such as addition of primers or positive control DNA.

Jena Bioscience’s lyophilized PCR mixes come in a number of formats including:

Amplification directly from whole blood or tissues

  • avoids any prior DNA purification/li>
  • minimizes amount of source material

Amplification of multiple fragments

  • optimized for multi-parallel target gene amplification in a single tube

Real-Time Master Lyophilisates

  • sample DNA quantification in broad dynamic range
  • exceptional sensitivity and selectivity

Taq and Hot Start Master Lyophilisates

  • robust, reliable results for all routine PCR
  • low background with hot start master mixes

Reverse Transcription Master Lyophilisates

  • engineered reverse transcriptase with enhanced thermal stability
  • increased specificity, high yield and efficiency for “difficult” (highly structured) cDNA fragments

Ready-to-Use Lyophilisates containing custom primers are custom made master mix lyophilisates comprising primers, enzyme, dNTPs and reaction buffer. Provide us with primer sequence and we deliver preloaded PCR tubes and plates ready-made for your special application. The only thing you still have to do is adding template RNA, filling up with water and starting the cycler!


Amplification Directly from Whole Blood or Tissues

Direct PCR Lyophilisate – Lyophilized Master Mix for direct PCR amplification from blood, animal and plant tissue
Direct PCR Lyophilisate is designed for PCR amplification directly from whole blood, animal tissues and plant tissues without the need of prior DNA purification processes. The robust enzyme in combination with a specially optimized unique buffer system resists various PCR inhibitors of blood and tissue samples.

Multiplex PCR Master Lyophilisate – Lyophilized Master Mix for multiplex PCR application
Multiplex PCR Master Lyophilisate is specially designed for the set-up of multiplex PCR reactions. It contains an optimized composition of polymerase, nucleotides, MgCl2 and stabilizing components in a specifically developed buffer system allowing the parallel amplification of a multitude of fragments in a single PCR assay.


Amplification of Multiple Fragments

Direct PCR Lyophilisate – Lyophilized Master Mix for direct PCR amplification from blood, animal and plant tissues
Direct PCR Lyophilisate is designed for PCR amplification directly from whole blood, animal tissues and plant tissues without the need of prior DNA purification processes. The robust enzyme in combination with a specially optimized unique buffer system resists various PCR inhibitors of blood and tissue samples.

Multiplex PCR Master Lyophilisate - Lyophilized Master Mix for multiplex PCR application
Multiplex PCR Master Lyophilisate is specially designed for the set-up of multiplex PCR reactions. It contains an optimized composition of polymerase, nucleotides, MgCl2 and stabilizing components in a specifically developed buffer system allowing the parallel amplification of a multitude of fragments in a single PCR assay.


Real-Time Master Lyophilisates

qPCR ProbesMaster Lyophilisate – Lyophilized real-time PCR Master Mix for dual labeled probes
qPCR ProbesMaster Lyophilisate is designed for quantitative real-time analysis of DNA samples using DNA probe based detection. The lyophilisate is recommended for use with Dual Labeled Fluorescent Probes, e.g. TaqMan®, Molecular Beacons or FRET probes.
The Master contains all reagents required for qPCR (except template, primer and labeled fluorescent probe).

qPCR GreenMaster Lyophilisate – Lyophilized real-time PCR Master Mix with green-fluorescent DNA stain
qPCR GreenMaster Lyophilisate is designed for quantitative real-time analysis of DNA samples using the fluorescent DNA stain EvaGreen®. The fluorescent dye intercalates into the amplification product during the PCR process and enables the rapid analysis of target DNA without the need of sequence-specific labeled probes.
The Master contains all reagents required for qPCR (except template and primer).


Taq and Hot Start Master Lyophilisates

Red Load Taq Master Lyophilisate – Lyophilized Taq Master Mix containing red gel loading dye
Red Load Taq Master Lyophilisate contains additionally an inherent red dye allowing the direct loading of the PCR reaction product onto the gel.
To perform PCR, fill up the vials with a premix of primers and PCR-grade water and add DNA template. If necessary, centrifuge to remove bubbles, vortex the vials to assure homogeneity and start cycling.

Taq Master Lyophilisate – Lyophilized Taq Master Mix
Taq Master Lyophilisate combines highest performance with convenience of use and stability.
To perform PCR, fill up the vials with a premix of primers and PCR-grade water and add DNA template. If necessary, centrifuge to remove bubbles, vortex the vials to assure homogeneity and start cycling.

Hot Start Master Lyophilisate – Lyophilized Hot Start Master Mix
Hot Start Master Lyophilisate provides improved specificity and sensitivity when amplifying low-copy-number targets in complex backgrounds. The polymerase activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation.


Reverse Transcription Master Lyophilisates

One-Step RT-PCR Master Lyophilisate – Lyophilized One-Step RT-PCR Master Mix
One-Step RT-PCR Master Lyophilisate is designed for performing maximum sensitive and specific RT-PCRs convenient in single tubes. The enzyme mix is based on a genetically engineered RT polymerase with enhanced thermal stability resulting in an increased specificity, higher cDNA yield and an improved efficiency for highly structured and long cDNA fragments.
The lyophilisate contains all reagents required for RT-PCR (except template and primer) in one tube to ensure fast and easy preparation with a minimum of pipetting steps. The premium quality enzyme mix, ultrapure dNTPs and the optimized complete reaction buffer ensure superior amplification results.

RT Master Lyophilisate – Lyophilized Master Mix for Reverse Transcription
RT Master Lyophilisate contains a genetically engineered version of M-MLV Reverse Transcriptase (M-MLV RT) with eliminated RNase H activity and increased thermal stability. The enzyme is a RNA-directed DNA polymerase that synthesizes a complementary DNA strand initiating from a primer using single-stranded RNA or DNA as template.
Each vial contains all components (except primers and template) required for a 20 µl reverse transcription assay. To perform the assay, only fill up the vials with a mix of primers and RNA template.


Where can I find more information about Jena Bioscience?

Visit the manufacturer page at www.stratech.co.uk/jena_bioscience, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


Product Focus: trFluor™ Kits – Time-Resolved Fluorometry (TRF)

Many biological compounds present in cells, serum or other biological fluids are naturally fluorescent, and thus the use of conventional, prompt fluorophores leads to serious limitations in assay sensitivity due to the high background caused by the autofluorescence of the biological molecules to be assayed. The use of long-lived fluorophores combined with time-resolved detection (a delay between excitation and emission detection) minimizes prompt fluorescence interferences.


ReadiLink™ trFluor™ Eu Antibody Labeling Kit *Microscale Optimized for Labeling 50 ug Antibody Per Reaction*

 

TR Fluor™ Eu probes enable time-resolved fluorometry (TRF) for assays that require high sensitivity. These TR Fluor™ Eu probes have large Stokes shifts and extremely long emission half-lives when compared to more traditional fluorophores such as Alexa Fluor or cyanine dyes. Compared to the other TRF compounds, our TR Fluor™ Eu probes have relatively high stability, high emission yield and ability to be linked to biomolecules. Moreover, our TR Fluor™ Eu probes are insensitive to fluorescence quenching when conjugated to biological polymers such as antibodies.

Detailed Information:   Protocol   References   MSDS   Spectrum


ReadiLink™ trFluor™ Tb Protein Labeling Kit *Microscale Optimized for Labeling 50 ug Antibody Per Reaction*

 

TR Fluor™ Tb probes enable time-resolved fluorometry (TRF) for assays that require high sensitivity. These TR Fluor™ Tb probes have large Stokes shifts and extremely long emission half-lives when compared to more traditional fluorophores such as Alexa Fluor or cyanine dyes. Compared to the other TRF compounds, our TR Fluor™ Tb probes have relatively high stability, high emission yield and ability to be linked to biomolecules. Moreover, our TR Fluor™ Tb probes are insensitive to fluorescence quenching when conjugated to biological polymers such as antibodies.

Detailed Information:   Protocol   References   MSDS   Spectrum


Where can I find more information about AAT Bioquest?

Visit the manufacturer page at www.stratech.co.uk/aatbioquest, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


 

Product Focus: AAT Bioquest’s mFluor™ dyes

AAT Bioquest’s mFluor™ dyes are developed for flow cytometry-focused applications. These dyes can be well excited by the laser lines of flow cytometers (e.g., 405 nm, 488 nm and 633 nm).

Detailed Information:   Protocol   References   MSDS   Spectrum

Catalogue No. Unit Product Name
1100 2 Labelings  ReadiLink™ mFluor™ Violet 450 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1105 2 Labelings  ReadiLink™ mFluor™ Violet 420 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1110 2 Labelings  ReadiLink™ mFluor™ Violet 510 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1114 2 Labelings  ReadiLink™ mFluor™ Violet 540 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1120 2 Labelings  ReadiLink™ mFluor™ Blue 570 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1123 2 Labelings  ReadiLink™ mFluor™ Green 620 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1126 2 Labelings  ReadiLink™ mFluor™ Yellow 630 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1130 2 Labelings  ReadiLink™ mFluor™ Red 700 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
1131 2 Labelings  ReadiLink™ mFluor™ Red 780 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

Where can I find more information about AAT Bioquest?

Visit the manufacturer page at www.stratech.co.uk/aatbioquest, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


 

Product Focus: Purmorphamine

Purmorphamine, which directly binds and activates Smoothened, blocks BODIPY-cyclopamine binding to Smo with IC50 of ~ 1.5 μM and also is an inducer of osteoblast differentiation with EC50 of 1 μM.

Purmorphamine Chemical Structure
Molecular Weight: 520.62


Biological Activity

Targets Smoothened
IC50 ~ 1.5 μM [1]
In vitro Purmorphamine activates the Hedgehog pathway by directly binds and activates Smoothened with IC50 of ~ 1.5 μM in compete with cyclopamine, a Smo antagonist. [1] Purmorphamine is a potent inducer of osteogenesis in multipotent C3H10T1/2 cells. The EC50 (based on ALP expression) for Purmorphamine is 1 μM in C3H10T1/2 cells. Purmorphamine (1 μM) and BMP-4 (100 ng/mL) together increase ALP activity more than 90-fold in 3T3-L1 cells. [2] In contrast to BMP-4, Purmorphamine induces osteogenesis by activating Hedgehog signaling in multipotent mesenchymal progenitor cells. [3]
In vivo Purmorphamine up-regulates ALP expression in human mesenchymal stem cell-based constructs on rats. [4]
Features

Protocol(Only for Reference)

Kinase Assay: [1]

Binding assay Smo binding assays are conducted with BODIPY-cyclopamine and Smo-overexpressing cells as previously described4,5, using CMV promoter-based, SV40 origin-containing expression constructs for Smo-Myc3, the deletion mutant Smo CRD (deletion of amino acids 68 to 182), and Smo CT (deletion of amino acids 556 to 793). HEK 293T cells are grown on poly-D-lysine-treated glass coverslips in 12-well plates until 70% confluency and then transfected with the appropriate expression construct (0.5 ug/well) using FuGene 6 according the manufacturer’s protocols. Two days after transfection, the HEK 293T cells are incubated with DMEM containing 0.5% bovine calf serum, 5 nM BODIPY-cyclopamine, and varying concentrations of Purmorphamine (0, 1.5, or 5 uM) (1 mL/well) for 1 h at 37 ℃. The Smo-overexpressing cells are then washed with 1 × PBS buffer (1 mL/well), mounted with DAPI-containing medium, and visualized using a Leica DM4500B fluorescence microscope. For binding assays using fixed cells, the Smo-overexpressing HEK 293T cells are fixed with 3% paraformaldehyde in 1 × PBS buffer for 10 min at room temperature (1 mL/well), treated with 1 × PBS containing 10 mM glycine and 0.2% sodium azide for 5 min (1 mL/well), washed with 1 × PBS buffer (1 mL/well), and treated with the Purmorphamine-containing media described above for 4 h at room temperature.

Cell Assay: [2]

Cell lines C3H10T1/2 cell
Concentrations 0.5-10 μM
Incubation Time 4 days
Method C3H10T1/2 cells are expanded in T175 flasks; cells at 13th passage are detached by trypsin/EDTA and diluted in the growth media. The resulting cell suspension is then plated into black clear bottom 384-well plates with 2500 cells/well in 100 µL growth medium using a Multi-dropTM liquid delivery system. After overnight incubation, cells attached to the bottom of the wells. A stock solution of each Purmorphamine in DMSO (500 nL) is delivered into corresponding well using a Mini TrakTM multiposition dispenser system to make a final concentration of 5μM of Purmorphamine. Cells are then incubated at 37℃ with 5% CO2 in air atmosphere. After 4 days, the medium is removed and 10 μL of passive lysis buffer is added into each well. After 5 min, 10 μL of alkaline phosphatase substrate solution is added to each well. After incubating 15 min at room temperature, the plates are read on an Acquest high-throughput plate reader following the manufacturer’s protocol.

References


Chemical Information

Download Purmorphamine SDF

Molecular Weight (MW) 520.62
Formula C31H32N6O2
CAS No. 483367-10-8
Storage 3 years -20℃ Powder
6 months-80℃ in DMSO
Synonyms
Solubility (25°C) * In vitro DMSO 4 mg/mL heating (7 mM)
Water <1 mg/mL (<1 mM)
Ethanol <1 mg/mL (<1 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Name 9H-Purin-6-amine, 9-cyclohexyl-N-[4-(4-morpholinyl)phenyl]-2-(1-naphthalenyloxy)-

Where can I find more information about Selleck Chemicals?

Visit the manufacturer page at www.stratech.co.uk/selleck_chemicals, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


 

Product Focus: PrecisionX™ CRISPR/Cas9 SmartNuclease™ System

Mammalian Genome Engineering with Guide RNAs


CRISPR/Cas9 SmartNuclease System

Genome Editing using guide RNAs
The recent discovery of the CRISPR/Cas9 complex has provided researchers an invaluable tool to target and modify any genomic sequence with high levels of efficacy and specificity. The system, consisting of an RNA-guided nuclease (Cas9) and guide RNA (gRNA) complementary to a target sequence, allows for sequence-specific cleavage of target loci across the genome.

  • Specific genome cleavage using gRNAs
  • Ready-to-transfect Cas9 mRNA available
  • All-in-one Cas9-gRNA vector options
  • Cas9 mutant Nickase vectors and mRNA
  • Easy and efficient gene knockouts


SBI’s PrecisionX™ Cas9 SmartNuclease™ system offers proven technology with unparalleled support and expertise to make your use of CRISPR/Cas9 easy and successful from the start.

Watch On-demand Cas9 Webinar.

SBI provides:

  • Detailed Application Notes for Gene Knock-Out, Editing, and Tagging applications; Custom gRNA and HR Donor Vector Design Services
  • Custom Genome Engineering Cloning & Cell Line Services
  • Complimentary Phone Consultation with our Cas9 Experts with every purchase
  • Live Technical Support
  • 3rd-party Validation of In Vitro and In Vivo Applications Using SmartNuclease Reagents

Expertise

Application Notes:
SBI’s genome engineering specialists have created detailed protocols for first-time and experienced users of the CRISPR/Cas9 system, including details on gRNA and HR Targeting Vector design for the following applications:

Custom Design Services:
Take advantage of SBI’s design expertise in genome engineering applications with their custom design services to have SBI design the optimal gRNA and/or HR Targeting vector sequences for your project.

Custom Cloning & Cell Line Services:
SBI also offers custom gRNA and HR donor vector cloning and cell line engineering services as a comprehensive solution for any genome engineering project.


Third-Party Validation

SBI was the first commercial company to offer CRISPR/Cas9 research tools, starting in April 2013, which means that many researchers have now produced successful results using SBI reagents in their labs. SBI’s Cas9 SmartNuclease™ reagents have been used successfully in both in vitro and in vivo applications, with product citations pending publication.

For example, up to a 75% mutation rate was recently achieved at the NIH using SBI’s Cas9 SmartNuclease system for direct genome editing in mouse zygotes, with 90% homozygous mutations (both alleles).


 

Background on How CRISPR/Cas9 Works

The recent discovery of the type II prokaryotic CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system, originally discovered in the bacterium Streptococcus pyogenes that works as a mechanism to defend against viruses and foreign DNA, has provided a revolutionary tool for targeted genome engineering with simple elegance.

The system uses a nuclease, CRISPR-associated (Cas9), that complexes with small RNAs as guides (gRNAs) to cleave DNA in a sequence-specific manner upstream of the protospacer adjacent motif (PAM) in any genomic location. The CRISPR-Cas9 system is comprised of separate guide RNAs known as the crRNA and tracrRNA. These two separate RNAs have been combined into a single RNA to enable site-specific mammalian genome cutting through the design of a short guide RNA. SBI has built a series of all-in-one CRISPR-Cas9 cloning vectors with different promoter options. These cloning vectors will express a human codon-optimized Cas9 nuclease as well as any gRNA sequence of your choice linked to the tracrRNA. Simply design the gRNA for the genome target DNA sequence and clone into the Cas9 SmartNuclease™ vector for efficient genome engineering. The PrecisionX™ Cas9 SmartNuclease™ Vector products are the subject of pending US and foreign patent applications.


Resources

Online Resources

Technical References


Products

PrecisionX™ Cas9 SmartNuclease RNA Products

Product Size Catalogue No.
Transfection-ready Cas9 SmartNuclease mRNA (Eukaryotic version) 20 ug CAS500A-1
Transfection-ready Cas9 SmartNuclease mRNA (Prokaryotic version) 20 ug CAS502A-1
Transfection-ready Cas9 SmartNickase mRNA (Eukaryotic Nickase mutant version) 20 ug CAS504A-1
Linearized T7 gRNA SmartNuclease vector 10 reactions CAS510A-1
T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents) 1 Kit CAS510A-KIT
Extra T7 gRNA PCR primer mix (5 uM) 50 assays CAS510A-PR
Transfection-ready Cas9 SmartNuclease AAVS1 gRNA 10 ug CAS520A-1
Transfection-ready hspCas9-T2A-GFP SmartNuclease mRNA (wildtype Cas9 with GFP marker) 10 ug CAS530G-1
Transfection-ready hspCas9-T2A-RFP SmartNuclease mRNA (wildtype Cas9 with RFP marker) 10 ug CAS531R-1
Transfection-ready hspCas9-nickase-T2A-GFP SmartNickase mRNA (Cas9 Nickase mutant with GFP marker) 10 ug CAS534G-1
Transfection-ready hspCas9-nickase-T2A-RFP SmartNickase mRNA (Cas9 Nickase mutant with RFP marker) 10 ug CAS535R-1
Transfection-ready mRNAExpress positive control GFP mRNA 20 ug MR700A-2
Transfection-ready mRNAExpress positive control RFP mRNA 20 ug MR800A-2

 

PrecisionX™ Cas9 SmartNuclease All-in-One Vectors

Product Size Catalogue No.
EF1-hspCas9-H1-gRNA linearized SmartNuclease vector 10 reactions CAS900A-1
CAG-hspCas9-H1-gRNA linearized SmartNuclease vector 10 reactions CAS920A-1
CMV-hspCas9-H1-gRNA linearized SmartNuclease vector 10 reactions CAS940A-1
MSCV-hspCas9-H1-gRNA linearized SmartNuclease vector 10 reactions CAS960A-1
PGK-hspCas9-H1-gRNA linearized SmartNuclease vector 10 reactions CAS980A-1
EF1-T7-hspCas9-T2A-GFP-H1-gRNA linearized SmartNuclease vector 10 reactions CAS700G-1
EF1-T7-hspCas9-T2A-RFP-H1-gRNA linearized SmartNuclease vector 10 reactions CAS701R-1
CAG-T7-hspCas9-T2A-GFP-H1-gRNA linearized SmartNuclease vector 10 reactions CAS720G-1
CAG-T7-hspCas9-T2A-RFP-H1-gRNA linearized SmartNuclease vector 10 reactions CAS721R-1
CMV-T7-hspCas9-T2A-GFP-H1-gRNA linearized SmartNuclease vector 10 reactions CAS740G-1
CMV-T7-hspCas9-T2A-RFP-H1-gRNA linearized SmartNuclease vector 10 reactions CAS741R-1
Cas9 SmartNuclease AAVS1-gRNA Targeting Vector [EF1a promoter] 10 ug CAS601A-1
Cas9 SmartNuclease AAVS1 Positive Control kit (includes CAS601A-1, EGIP 293T reporter line, AAVS1/GFP rescue donor, and primers for Surveyor assay) 1 Kit CAS605A-1

 

PrecisionX™ Cas9 SmartNickase Mutant All-in-One Vectors

Product Size Catalogue No.
Cas9 Nickase: EF1-hspCas9-nickase-H1-gRNA linearized SmartNickase vector 10 reactions CAS800A-1
Cas9 Null Nuclease: EF1-hspCas9-DM-H1-gRNA linearized NullNuclease vector 10 reactions CAS805A-1
Cas9 Nickase: CAG-hspCas9-nickase-H1-gRNA linearized SmartNickase vector 10 reactions CAS820A-1
Cas9 Nickase: CMV-hspCas9-nickase-H1-gRNA linearized SmartNickase vector 10 reactions CAS840A-1
Cas9 Nickase: EF1-T7-hspCas9-nickase-T2A-GFP-H1-gRNA linearized SmartNickase vector 10 reactions CAS750G-1
Cas9 Nickase: EF1-T7-hspCas9-nickase-T2A-RFP-H1-gRNA linearized SmartNickase vector 10 reactions CAS751R-1
Cas9 Nickase: CAG-T7-hspCas9-nickase-T2A-GFP-H1-gRNA linearized SmartNickase vector 10 reactions CAS770G-1
Cas9 Nickase: CAG-T7-hspCas9-nickase-T2A-RFP-H1-gRNA linearized SmartNickase vector 10 reactions CAS771R-1
Cas9 Nickase: CMV-T7-hspCas9-nickase-T2A-GFP-H1-gRNA linearized SmartNickase vector 10 reactions CAS790G-1
Cas9 Nickase: CMV-T7-hspCas9-nickase-T2A-RFP-H1-gRNA linearized SmartNickase vector 10 reactions CAS791R-1

 

PrecisionX™ Related Products

Product Size Catalogue No.
AAVS1 Safe Harbor cDNA/miRNA HR Donor Vector [pAAVS1D-PGK.MCS-EF1a.copGFPpuro] 10 ug GE602A-1
AAVS1 Safe Harbor Positive Control HR Donor Vector [pAAVS1D-CMV.RFP-EF1a.copGFPpuro] 10 ug GE603A-1
5′ and 3′ AAVS1 Positive Control Donor Vector Primer Mixes for Junction PCR Assays (10 uM) 100 ul each GE603PR-1
Basic HR Targeting Vector [MCS1-LoxP-MCS2-MCS3-pA-LoxP-MCS4] for Gene Knock-In/Out 10 ug HR100PA-1
Gene Knock-Out HR Targeting Vector [MCS1-EF1?-RFP-T2A-Puro-pA-MCS2] 10 ug HR110PA-1
GFP-Fusion HR Targeting Vector [GFP-pA-LoxP-EF1?-RFP-T2A-Puro-pA-LoxP-MCS] 10 ug HR120PA-1
T2A-GFP Co-Expression HR Targeting Vector [T2A-GFP-pA-loxP-EF1?-RFP-T2A-Puro-pA-LoxP-MCS] 10 ug HR130PA-1
GFP-T2A-Luciferase Co-Expression HR Targeting Vector [GFP-T2A-Luc-pA-loxP-EF1?-RFP-T2A-Puro-pA-LoxP-MCS] 10 ug HR150PA-1
IRES-GFP Co-Expression HR Targeting Vector [IRES-GFP-pA-loxP-MCS1-EF1?-RFP-T2A-Puro-pA-LoxP-MCS2] 10 ug HR180PA-1
Gene Knock-Out HR Targeting Vector with TK selection [MCS1-LoxP-EF1a-GFP-T2A-Puro-P2A-hsvTK-pA-LoxP-MCS2] 10 ug HR210PA-1
piggyBac HR Vector (5′ MCS-EF1-GFP-Puro-TK-3′ MCS) 10ug PBHR100A-1

Where can I find more information about SBI?

Visit the manufacturer page at www.stratech.co.uk/SBI, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


 

Product Focus: iFluor™ Antibody Labelling Kits

AAT Bioquest’s iFluor™ dyes are optimized for labeling proteins, in particular, antibodies.   These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm).

List of all available kits


ReadiLink™ iFluor™ 350 Antibody Labelling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

iFluor™ 350 dyes have fluorescence excitation and emission maxima of ~350 nm and ~440 nm respectively. These spectral characteristics make them an excellent replacement for AMCA and Alexa Fluor® 350 labeling dye (Alexa Fluor® is the trademark of Invitrogen). ReadiLink™ labeling kits essentially only require 2 simple mixing steps without a column purification needed. iFluor™ 350 SE used in this ReadiLink™ kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labeling ~2×50 ug antibody. Each of the two vials of iFluor™ 350 dye provided in the kit is optimized for labeling ~50 µg antibody. iFluor™ 350 SE protein labeling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor™ 350 SE.

Detailed Information:   Protocol   References   MSDS   Spectrum


 ReadiLink™ iFluor™ 488 Antibody Labelling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

Although FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence. Protein conjugates prepared with iFluor™ 488 dyes (Ex/Em=497 nm/525 nm) are far superior compared to conjugates of fluorescein derivatives such as FITC. iFluor™ 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of iFluor™ 488 is not affected by pH (4-10). This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. ReadiLink™ labeling kits essentially only require 2 simple mixing steps without a column purification needed. iFluor™ 488 SE used in this ReadiLink™ kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labeling ~2×50 ug antibody. Each of the two vials of iFluor™ 488 dye provided in the kit is optimized for labeling ~50 µg antibody. iFluor™ 488 SE protein labeling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor™ 488 SE.

Detailed Information:   Protocol   References   MSDS   Spectrum


ReadiLink™ iFluor™ 555 Antibody Labelling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

iFluor™ 555 dyes have fluorescence excitation and emission maxima of ~550 nm and ~570 nm respectively. iFluor™ 555 family has the spectral properties essentially identical to those of Cy3® (Cy3® is the trademark of GE Healthcare). Compared to Cy3 probes iFluor™ 555 family has much stronger fluorescence and higher photostability. Their fluorescence is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family a superior alternative to Cy3®. iFluor™ 555 family has become an excellent replacement for Cy3 and Alexa Fluor® 555 labeling dye (Alexa Fluor® is the trademark of Invitrogen). ReadiLink™ labeling kits essentially only require 2 simple mixing steps without a column purification needed. iFluor™ 555 SE used in this ReadiLink™ kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labeling ~2×50 ug antibody. Each of the two vials of iFluor™ 555 dye provided in the kit is optimized for labeling ~50 µg antibody. iFluor™ 555 SE protein labeling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor™ 555 SE.

Detailed Information:   Protocol   References   MSDS   Spectrum


 ReadiLink™ iFluor™ 594 Antibody Labelling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

iFluor™ 594 dyes have fluorescence excitation and emission maxima of ~590 nm and ~610 nm respectively. iFluor™ 594 family has the spectral properties similar to those of Texas Red® and Alexa Fluor® 594 (Texas Red® and Alexa Fluor® 594 are the trademarks of Invitrogen). iFluor™ 594 family is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family an excellent alternative to Texas Red® and Alexa Fluor® 594.Compared to Texas Red®, iFluor™ 594 is much easier to be conjugated with RPE with much higher conjugation yield, and the resulted RPE-iFluor™ 594 tandem has better FRET efficiency.ReadiLink™ labeling kits essentially only require 2 simple mixing steps without a column purification needed. iFluor™ 594 SE used in this ReadiLink™ kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labeling ~2×50 ug antibody. Each of the two vials of iFluor™ 594 dye provided in the kit is optimized for labeling ~50 µg antibody. iFluor™ 594 SE protein labeling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor™ 594 SE.

Detailed Information:   Protocol   References   MSDS   Spectrum


ReadiLink™ iFluor™ 633 Antibody Labelling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

iFluor™ 633 dyes have fluorescence excitation and emission maxima close to ~633 nm and ~655 nm respectively with good photostability. Our in-house comparable studies indicated that our iFluor™ 633 dyes are significantly brighter than the corresponding Cy5®. These spectral characteristics make them a superior alternative to Cy5® and Alexa Fluor®633 (Cy5® and Alexa Fluor® are the trademarks of GE Healthcare and Invitrogen respectively). iFluor™ 633 conjugates have been widely used in fluorescence animal imaging applications. ReadiLink™ labeling kits essentially only require 2 simple mixing steps without a column purification needed. iFluor™ 633 SE used in this ReadiLink™ kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labeling ~2×50 ug antibody. Each of the two vials of iFluor™ 633 dye provided in the kit is optimized for labeling ~50 µg antibody. iFluor™ 633 SE protein labeling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor™ 633 SE.

Detailed Information:   Protocol   References   MSDS   Spectrum


ReadiLink™ iFluor™ 647 Antibody Labelling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

iFluor™ 555 dyes have fluorescence excitation and emission maxima of ~550 nm and ~570 nm respectively. iFluor™ 647 family has the spectral properties essentially identical to those of Cy5® (Cy5® is the trademark of GE Healthcare). Compared to Cy5 probes iFluor™ 647 family has much stronger fluorescence and higher photostability. Their fluorescence is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family a superior alternative to Cy5®. iFluor™ 647 family has become an excellent replacement for Cy5 and Alexa Fluor® 647 labeling dye (Alexa Fluor® is the trademark of Invitrogen). ReadiLink™ labeling kits essentially only require 2 simple mixing steps without a column purification needed. iFluor™ 647 SE used in this ReadiLink™ kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labeling ~2×50 ug antibody. Each of the two vials of iFluor™ 647 dye provided in the kit is optimized for labeling ~50 µg antibody. iFluor™ 647 SE protein labeling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor™ 647 SE.

Detailed Information:   Protocol   References   MSDS   Spectrum


ReadiLink™ iFluor™ 680 Antibody Labelling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

iFluor™ 680 dyes have fluorescence excitation and emission maxima close to 680 nm and 700 nm respectively with good photostability. These spectral characteristics make them an excellent alternative to Cy5.5®, IRDye® 700 and Alexa Fluor® 680 (Cy5.5®, IRDye® and Alexa Fluor® are the trademarks of GE Healthcare, Li-COR and Invitrogen respectively). ReadiLink™ labeling kits essentially only require 2 simple mixing steps without a column purification needed. iFluor™ 680 SE used in this ReadiLink™ kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labeling ~2×50 ug antibody. Each of the two vials of iFluor™ 680 dye provided in the kit is optimized for labeling ~50 µg antibody. iFluor™ 680 SE protein labeling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor™ 680 SE.

Detailed Information:   Protocol   References   MSDS   Spectrum


 ReadiLink™ iFluor™ 700 Antibody Labelling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

iFluor™ 700 dyes have fluorescence excitation and emission maxima close to 690 nm and 710 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 700 (Alexa Fluor® is the trademark of Invitrogen). ReadiLink™ labeling kits essentially only require 2 simple mixing steps without a column purification needed. iFluor™ 700 SE used in this ReadiLink™ kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labeling ~2×50 ug antibody. Each of the two vials of iFluor™ 700 dye provided in the kit is optimized for labeling ~50 µg antibody. iFluor™ 700 SE protein labeling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor™ 700 SE.

Detailed Information:   Protocol   References   MSDS   Spectrum


ReadiLink™ iFluor™ 750 Antibody Labelling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

iFluor™ 750 dyes have fluorescence excitation and emission maxima close to 750 nm and 780 nm respectively with good photostability. Our in-house comparable studies indicated that our iFluor™ 750 dyes are significantly brighter than the corresponding Cy7® and Alexa Fluor® 750. These spectral characteristics make them a superior alternative to Cy7® and Alexa Fluor® 750 (Cy7® and Alexa Fluor® are the trademarks of GE Healthcare and Invitrogen respectively). iFluor™ 750 conjugates have been widely used in fluorescence animal imaging applications. ReadiLink™ labeling kits essentially only require 2 simple mixing steps without a column purification needed. iFluor™ 750 SE used in this ReadiLink™ kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labeling ~2×50 ug antibody. Each of the two vials of iFluor™ 750 dye provided in the kit is optimized for labeling ~50 µg antibody. iFluor™ 750 SE protein labeling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor™ 750 SE.

Detailed Information:   Protocol   References   MSDS   Spectrum


 ReadiLink™ iFluor™ 790 Antibody Labelling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*

iFluor™ 790 dyes have fluorescence excitation and emission maxima close to 790 nm and 810 nm respectively. These spectral characteristics make them an excellent alternative to IRDye® 800 (IRDye® is the trademark of Li-COR). Our in-house comparable studies indicated that our iFluor 790 dyes are significantly brighter than the corresponding IRDye® 800. iFluor™ 790 conjugates have been widely used in fluorescence animal imaging applications. ReadiLink™ labeling kits essentially only require 2 simple mixing steps without a column purification needed. iFluor™ 790 SE used in this ReadiLink™ kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labeling ~2×50 ug antibody. Each of the two vials of iFluor™ 790 dye provided in the kit is optimized for labeling ~50 µg antibody. iFluor™ 790 SE protein labeling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor™ 790 SE.

Detailed Information:   Protocol   References   MSDS   Spectrum


Where can I find more information about AAT Bioquest?

Visit the manufacturer page at www.stratech.co.uk/aatbioquest, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


 

 


Product Focus: CLICKable FLAG-tag

FLAG-tag is a small and hydrophilic affinity tag (DYKDDDDK) that is widely used for antibody-mediated purification and immunological detection (Western Blot, ELISA, FACS, immunohistochemistry) of recombinant proteins[1-3] due to

  • its minimal interference with protein function
  • the possibility of competitive elution under non-denaturing conditions with a FLAG peptide excess

So far, the introduction of a FLAG-tag has been limited to recombinant proteins only: The gene encoding the protein of interest needs to be cloned into a FLAG-tag vector followed by the expression of the FLAG-fusion protein in host cells (e.g. E. coli, S. cerevisiae, and mammalian cells).

The novel compounds DBCO-PEG4-FLAG and Azide-PEG3-FLAG now enable FLAG-tag attachment to any Azide-, terminal Alkyne- or Cyclooctyne (e.g. DBCO*)-tagged molecule via Click Chemistry (Fig. 1). FLAG-tag functionalized molecules are subsequently detected by an anti-FLAG antibody that is either immobilized onto a matrix (for purification) or coupled with a fluorescent dye or reporter enzyme for direct or indirect detection, respectively[2,3].

*DBCO = Dibenzocyclooctyne (also known as ADIBO = Azadibenzocyclooctyne)

Figure 1: FLAG-tag labeling of Azide-tagged (A) and terminal Alkyne- or DBCO-tagged molecules (B). Molecule solubility and efficient FLAG-tag detection is ensured by the integrated PEG-linker.
SPAAC = Cu(I)-free strain promoted Alkyne-Azide Click Chemistry. CuAAC = Cu(I)- catalyzed terminal Alkyne-Azide Click Chemistry.


Selected References

  1. Hopp et al. (1988) A Short Polypeptide Marker Sequence Useful for Recombinant Protein Identification and Purification. Bio/Technology 6 (19):1204.
  2. Einhauer et al. (2001) The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins. J. Biochem. Biophys. Methods 49:455.
  3. Brizzard et al. (1997) Epitope tagging of recombinant proteins. Current Protocols in Neuroscience:5.8.1.

Where can I find more information about Jena Bioscience?

Visit the manufacturer page at www.stratech.co.uk/jena_bioscience, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


 

NEW Products: Purified Exosomes, Dexosomes and Exo-Fect Kits

Purified Human Exosomes and Mouse Dexosomes

Exosomes are 60 – 180 nm membrane vesicles secreted by most cell types in vivo and in vitro and contain distinct subsets of RNAs and proteins depending upon the cell type from which they are secreted, making them useful for biomarker discovery and functional characterization. Exosomes are nano-sized shuttles that transport signaling RNAs, lipids and proteins to other cells. Studying exosome contents are thus a “liquid biopsy” for biomarkers to gain insights into their roles in disease initiation and progression.

What can I do with purified exosomes?

PC-3 NanoSight Exosomes

PC-3 NanoSight

  • Use highly purified exosomes for standards
  • Utilize for protein, and RNA cargo studies
  • Investigate Human Mesenchymal stem cell exosomes for activity in vivo
  • Engineer the Murine dendritic cell dexosomes as RNA/protein
    delivery vehicles

 

How the exosomes are prepared

  • All cell lines grown in exosome-depleted FBS (Exo-FBS)
  • Exosomes purified using ExoQuick-TC
  • Characterized by NanoSight for size and intactness
  • CD63 Western blot analysis verified
  • Exosomes provided at >1×10^6 exosomes (50 ug protein)
  • Exosomes are fully functional

 


Cancer, Stem cells and Exosomes

Tumor-secreted exosomes have intact and functional mRNAs, small RNAs, and proteins that can alter the cellular environment to favor tumor growth. Tumor metastasis involves manipulation of the tumor’s microenvironment to optimize conditions for survival and growth both locally and to begin seeding for expansion into distant sites. Potential locations for remote tumor implantation are thus prepared in advance of actual metastasis through exosomal intercellular communications. SBI has purified exosomes from numerous, model human cancer cell lines in culture to accelerate the studies of their signaling contents and how these tumor exosomes function.

Mesenchymal stem cells (MSCs) and their exosomes are the stem cell of choice for regenerative medicine and their exosomes are known to promote tissue injury repair and reduce inflammatory responses. There is great interest in exploring the cargo of MSC exosomes and engineering them for potential therapeutic delivery applications. SBI offers highly purified exosomes from human bone marrow and pre-adipocyte MSCs for advancing the research of MSC-derived exosomes. For use in animal models, murine bone marrow-derived immature dendritic cell line exosomes, called “dexosomes”, are also available. These exosomes can be engineered as delivery shuttles in vivo after transfecting them with exogenous RNAs, and can even be used for shuttling some drugs to target cells.


Products

Purified Human and Mouse Exosomes

Product Size Catalogue No.
MCF-7 Human breast cancer, noninvasive cell line: >1×10^6 frozen exosomes 50 ug EXOP-100A-1
MDA-MB-231 Human breast cancer, aggressive/invasive/metastatic cell line: >1×10^6 frozen exosomes 50 ug EXOP-105A-1
PC-3 Human prostate cancer cells derived from metastatic cancer cell line: >1×10^6 frozen exosomes 50 ug EXOP-115A-1
A549 Human non-small cell lung cancer cell line: >1×10^6 frozen exosomes 50 ug EXOP-120A-1
H841 Human small cell lung cancer cell line: >1×10^6 frozen exosomes 50 ug EXOP-125A-1
H196 Human small cell lung cancer cell line: >1×10^6 frozen exosomes 50 ug EXOP-130A-1
DMS114 Human small cell lung cancer cell line: >1×10^6 frozen exosomes 50 ug EXOP-135A-1
PCS-500-011 Human pre-adipose derived mesenchymal stem cell: >1×10^6 frozen exosomes 50 ug EXOP-140A-1
PCS-500-012 Human bone marrow-derived mesenchymal stem cell line: >1×10^6 frozen exosomes 50 ug EXOP-145A-1
HEK293 Human embryonic kidney cell line: >1×10^6 frozen exosomes 50 ug EXOP-110A-1
JAWSII Mouse bone marrow immature dendritic cell line: >1×10^6 frozen dexosomes 50 ug EXOP-200A-1
Human pooled serum: >1×10^6 frozen exosomes 50 ug EXOP-300A-1

Exo-Fect Exosome Transfection Kits

Product Size Catalogue No.
Exo-Fect Exosome Transfection Kit 10 reactions EXFT10A-1
Exo-Fect Exosome Transfection Kit 20 reactions EXFT20A-1

 

ExoQuick and ExoQuick-TC Products

Product Size Catalogue No.
ExoQuick serum exosome precipitation solution (5 ml) 75 reactions EXOQ5A-1
ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL), replaces EXOQ5TD-1 product 75 reactions EXOQ5TM-1
Thrombin Plasma prep for Exosome precipitation (500 ul at 500U/mL), replaces TDEXO-1 product 100 reactions TMEXO-1
ExoQuick serum exosome precipitation solution (20 ml) 300 reactions EXOQ20A-1
ExoQuick-TC for Tissue Culture Media and Urine (10ml) 10 reactions EXOTC10A-1
ExoQuick-TC for Tissue Culture Media and Urine (50ml) 50 reactions EXOTC50A-1
Exosome-depleted FBS Media Supplement – USA Certified 50 ml EXO-FBS-50A-1
Exosome-depleted FBS Media Supplement – USA Certified 250 ml EXO-FBS-250A-1
ExoQuick-LP Lipoprotein Pre-Clear & Exosome Precipitation Kit 5 reactions EXOLP5A-1

The ExoQuick serum reaction size is based on using 250 µl serum for exosome isolation and ExoQuick-LP is based on a 100 µl starting serum sample.
The ExoQuick-TC reagent provides enough for either 10 reactions (EXOTC10A-1) or 50 reactions (EXOTC50A-1) from 5 ml Media or Urine for exosome isolation.

 

 

Exo-FBS Ordering

Product Size Catalogue No.
Exosome-depleted FBS Media Supplement – USA Certified 50 ml EXO-FBS-50A-1
Exosome-depleted FBS Media Supplement – USA Certified 250 ml EXO-FBS-250A-1
Exosome-depleted FBS Media Supplement Heat Inactivated – USA Certified 50 ml EXO-FBSHI-50A-1
Exosome-depleted FBS Media Supplement Heat Inactivated – USA Certified 250 ml EXO-FBSHI-250A-1
ExoQuick-TC for Tissue Culture Media and Urine (10ml) 10 reactions EXOTC10A-1
ExoQuick-TC for Tissue Culture Media and Urine (50ml) 50 reactions EXOTC50A-1

Use ExoQuick-TC with Exo-FBS media to isolate exosomes being secreted from your cells in culture.

 

Exosome Antibodies, Arrays and ELISA Kits

Product Size Catalogue No.
Anti-CD63 Antibody (rabbit anti-human, mouse, rat, horse) with goat anti-rabbit HRP secondary antibody 25 ul EXOAB-CD63A-1
Anti-CD81 Antibody (rabbit anti-human, mouse, rat) with goat anti-rabbit HRP secondary antibody 25 ul EXOAB-CD81A-1
Anti-CD9 Antibody (rabbit anti-human, mouse, rat) with goat anti-rabbit HRP secondary antibody 25 ul EXOAB-CD9A-1
Anti-Hsp70 Antibody (rabbit anti-human, mouse, rat) with goat anti-rabbit HRP secondary antibody 25 ul EXOAB-Hsp70A-1
ExoAb Antibody Kit (CD9, CD63, CD81, Hsp70 antibodies, rabbit anti-human, mouse, rat) with goat anti-rabbit HRP secondary antibody 25 ul each EXOAB-KIT-1
Exo-Check Exosome Antibody Array (4 arrays) 4 Arrays EXORAY-4
Exo-Check Exosome Antibody Array (8 arrays) 8 Arrays EXORAY-8
Exo-Check Exosome Antibody Array (12 arrays) 12 Arrays EXORAY-12
Exosome ELISA Complete Kit (CD63 detection) 96 reactions EXOEL-CD63A-1
Exosome ELISA Complete Kit (CD81 detection) 96 reactions EXOEL-CD81A-1
Exosome ELISA Complete Kit (CD9 detection) 96 reactions EXOEL-CD9A-1
Human Exosome lysate positive protein control (10ug/ul) 50 ul EXOAB-POS-1
Exosome binding buffer for ELISAs 20 ml EXOEL-BB-20
Exosome calibration ELISA standards for CD9, CD63 and CD81 ExoELISAs (15 standard curves) 1 ml EXOEL-SD-1

 


Resources

Online Resources

Technical references and product citations

  1. Bo Yu, Xiaomin Zhang and Xiaorong Li. Exosomes Derived from Mesenchymal Stem Cells. Int. J. Mol. Sci. 2014, 15, 4142-4157.
  2. Drake RR, Kislinger T. The proteomics of prostate cancer exosomes. Expert Rev Proteomics. 2014 Feb 25. [Epub ahead of print].
  3. Xiao X, Yu S, Li S, Wu J, Ma R, et al. Exosomes: Decreased Sensitivity of Lung Cancer A549 Cells to Cisplatin. PLoS ONE 9(2): e89534.
  4. Feliciano DM, Zhang S, Nasrallah CM, Lisgo SN, Bordey A. Embryonic Cerebrospinal Fluid Nanovesicles Carry Evolutionarily Conserved Molecules and Promote Neural Stem Cell Amplification. PLoS One. 2014 Feb 12;9(2):e88810.
  5. McCready J, Sims JD, Chan D, Jay DG.. Secretion of extracellular hsp90alpha via exosomes increases cancer cell motility: a role for plasminogen activation. BMC Cancer. 2010 Jun 16;10:294.

Where can I find more information about SBI?

Visit the manufacturer page at www.stratech.co.uk/SBI, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.