Supplier Focus: Jena Bioscience – Epigenetics, Direct 5hmC detection

5-Hydroxymethyl-2′-deoxycytidine (5hmC), known as “the sixth base of DNA”, is produced by the Tet family Fe(II)-dependent oxygenases, which catalyze the oxidation of 5-Methyl-2′-deoxycytidine (5mC) to 5hmC. The conversion of 5mC into 5hmC is assumed to be the first step in a pathway leading towards DNA demethylation[1]. The functional role of 5hmC in mammalian genomes is still unclear but it is known that 5hmC is critical for brain development and loss of 5hmC levels has been associated with leukemia and melanoma[2,3,4].

Oxidative bisulfite sequencing (oxBS-seq)[5] and Tet-assisted bisulfite sequencing (TAB-Seq)[6] are currently used for whole genome mapping of 5hmC at single base resolution. Both techniques are expensive and require special facilities with experienced personnel. Shahal et al. however, recently reported a high throughput method for simple direct optical detection of 5hmC content in genomic DNA making use of Click Chemistry with DBCO-CY5 (Figure 1)[7].

Figure 1: 5hmC labeling procedure according to Shahal et al.[7]. In the first step, β-GT catalyzes the conversion of 5hmC to 6-azide-β-glucosyl-5-hydroxymethyl-cytidine (N3-5-gmC) using the synthetic cofactor Uridine-diphosphate-6-azide-glucose (UDP-6-N3-Glu). In the second step, the azide group is fluorescently labeled by the reactive alkyne DBCO-CY5 via Copper-free Click Chemistry, forming fluorescently tagged 5hmC. Fluorescence intensity of the resulting DNA product is directly proportional to the number of 5hmC residues. Two cytidine analogs were used for control experiments, 5-Propargylamino-dCTP-Cy5 for the Cy5-73 bp controls and 5-Hydroxymethyl-dCTP for 5hmC-73 bp standards.

Jena Bioscience offer a selection of epigenetic tools:

  • Carboxy Cytidine (caC):
    • 5-Carboxy-2′-deoxycytidine (N-1067)
  • Click Chemistry Reagents
  • Formyl Cytidine (fC):
    • 5-Formyl-2′-deoxycytidine (N-1069)
  • Hydroxymethyl Cytidines (5hmC):
    • 5-Hydroxymethyl-2′-deoxycytidine (N-1070)
    • 5-Hydroxymethyl-dCTP (NU-932)
  • Methyl Cytidines (mC):

Selected References

  1. Münzel et al. (2011) 5-Hydroxymethylcytosin, die sechste Base des Genoms. Angew. Chem. 123:6588.
  2. Tahiliani et al. (2009) Conversion of 5-methylcytosine to 5-hydroxymethylcytosine in mammalian DNA by MLL partner TET1. Science 324 (5929):930.
  3. Lian et al. (2012) Loss of 5-hydroxymethylcytosine is an epigenetic hallmark of melanoma. Cell 150 (6):1135.
  4. Ko et al. (2010) Impaired hydroxylation of 5-methylcytosine in myeloid cancers with mutant TET2. Nature 468 (7325):839.
  5. Booth et al. (2013) Oxidative bisulfite sequencing of 5-methylcytosine and 5-hydroxymethylcytosine. Nat. Protoc. 8 (10):1841.
  6. Booth et al. (2012) Quantitative sequencing of 5-methylcytosine and 5-hydroxymethylcytosine at single-base resolution. Science 336 (6083):934.
  7. Shahal et al. (2014) Spectroscopic quantification of 5-Hydroxymethylcytosine in genomic DNA. Anal. Chem. 86:8231.

Where can I find more information about Jena Bioscience?

Visit the manufacturer page at www.stratech.co.uk/jena_bioscience, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


Product Focus: Firefly Luciferase Substrate

Signosis has developed Firefly Luciferase Substrate for detecting firefly luciferase activity in their luciferase reporter product lines, including stable cell lines, TF reporter plasmids, and our Bac-In reporter systems, as well as third-parties firefly reporters in the market.  This affordable and cost-effective reagent produces a strong light signal that is comparable to competitors’ substrates.

Manual

Benefits

  • High signal and responsive – Produces strong and stable light signal.
  • Economical – Less than $0.20 per reaction.
  • Compatible – Works with all Signosis luciferase products and third-party firefly luciferase systems.

HepG2 cells were transduced with Bac-In CMV-Luciferase for 24 hours and cells were lysed to assay for luciferase signal with the Signosis Firefly Luciferase Substrate. Robust luciferase signal was detected after initial luminometer reading. The signal showed little decay at 5 minutes post substrate addition and 80% of luciferase signal remained after 10 minutes.


Where can I find more information about Signosis?

Visit the manufacturer page at www.stratech.co.uk/signosis, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


Supplier Focus: Creative Bioarray – Stem Cell Offering

Creative Bioarray offers over 1,000 stem cell products including adult stem cells, tumour stem cells, embryonic stem cells, iPS cells and other stem cells.

Adult Stem Cells are there to repair any damage that has been done to tissue and rebuild it back to its original state. We have many samples from different organs and systems of mice and rats.

Tumour Stem Cells samples are extremely valuable to those who need to compare them to normal stem cells of a certain origin and study how they give rise to cancerous cells.

Embryonic Stem Cells are available for both human and mouse embryos.

iPS Cells are cells that are derived from adult cells and are extremely useful in modeling diseases and drug development.


Adult Stem Cells

Adult stem cells are undifferentiated cells, found throughout the body after development that multiply by cell division to replenish dying cells and regenerate damaged tissues. Also known as somatic stem cells, they can be found in juvenile as well as adult animals and human bodies. Creative Bioarray provides adult stem cells from various tissue/organs of human, mouse and rat.

  • Human Adult Stem Cells
  • Mouse Adult Stem Cells
  • Rat Adult Stem Cells
  • Dog Adult Stem Cells
  • Rabbit Adult Stem Cells

Tumour Stem Cells

Tumour stem cells are cancer cells (found within tumors or hematological cancers) that possess characteristics associated with normal stem cells, specifically the ability to give rise to all cell types found in a particular cancer sample. Creative Bioarray offers tumor stem cells representing more than 20 types of carcinoma.

Embryonic Stem Cells

Embryonic stem cells (ES cells) are pluripotent stem cells derived from the inner cell mass of a blastocyst, an early-stage preimplantation embryo. Creative Bioarray provides embryonic stem cells from both human and mouse.

  • Human Embryonic Stem Cells
  • Mouse Embryonic Stem Cells
  • Rat Embryonic Stem Cells

iPSC

Induced pluripotent stem cells (also known as iPS cells or iPSC) are a type of pluripotent stem cell that can be generated directly from adult cells. We offer human iPSC lines derived from various primary cells by different inducing methods.

Other Stem Cells

Creative Bioarray also provides cells differentiated from stem cells.


Where can I find more information about Creative Bioarray?

Visit the manufacturer page at www.stratech.co.uk/creative-bioarray, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


Supplier Focus: Biotool – cDNA Synthesis SuperMix

Biotool offers a range of optimized cDNA synthesis systems (SuperMix) to generate cDNA for a variety of research interests.

Features All-in-One cDNA Synthesis SuperMix SuperFast cDNA Synthesis SuperMix SuperZyme III cDNA Synthesis SuperMix ThermoStable cDNA Synthesis SuperMix
RT-PCR
qRT-PCR
Enzyme activity temperature 42 ºC 42 ºC 50 ºC 65 ºC
RT extension time 15-30 mins 5 mins 30 mins 30 mins
Maximal cDNA length ≤ 12 kb ≤12 kb ≤ 15 kb ≤ 20 kb
gDNA remover
RNase H deficiency
Application cDNA Synthesis for real time-PCR assay extremely fast cDNA Synthesis (cDNA product is suitable for routine RT-PCR and qPCR) 3′ and 5′ RACE;cDNA library construction;cDNA synthesis (especially for GC-rich RNA templates or RNA templates of complex secondary structure) Full-length cDNA library construction;cDNA synthesis (especially for GC-rich RNA templates or RNA templates of complex secondary structure)

All-in-One cDNA Synthesis SuperMix

BioScript All-in-One cDNA Synthesis SuperMix provides first-strand cDNA synthesis, optimized with the high-temperature capability of Reverse Transcriptase and an optimized SuperMix formula. In one tube, the pre-blended 5× qRT SuperMix contains all the necessary components for reverse transcription of YOUR RNA template. The resulting cDNA product is suitable for use in real-time quantitative RT-PCR (qRT-PCR).

User Guide

  • Reproducibility: Modified Oligo dT: Random primers ratio facilitates qPCR repeatability.
  • High compatibility: cDNA product is suitable for application with SYBR Green or Probe qPCR.


SuperFast cDNA Synthesis SuperMix

SuperFast cDNA Synthesis SuperMix is designed for cDNA synthesis by genetic modification. Reverse transcription can be completed within 5 minutes, due to high affinity with RNA templates and a fast extension rate. The cDNA is efficiently synthesized by incubating the SuperFast RT/RI Enzyme Mix with the optimized 2 × TS SuperFast Reaction Mix. The cDNA products are suitable for downstream PCR and qPCR applications.

User Guide

  • Fast synthesis: Only 5 minutes needed for reverse transcription.
  • Higher yield: Save RNA from degradation due to the fastest reverse transcription.
  • Size of cDNA: Up to 12 kb in product length, more full-length cDNA is obtained.


SuperZyme III cDNA Synthesis SuperMix

SuperZyme III cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA. The cDNA is efficiently synthesized by employing SuperZyme III RT/RI Enzyme Mix and an optimized 2×TS III Reaction Mix. RNase H-Deficiency reduces RNA template degradation during the 1st-strand cDNA synthesis. Product lengths of cDNA can be up to 15 kb. The cDNA products are suitable for downstream PCR, qPCR and RNA-sequencing applications.

User Guide


ThermoStable cDNA Synthesis SuperMix

ThermoStable cDNA Synthesis SuperMix (+gDNA Remover) is engineered to have high thermal stability (up to 65°C) for efficient transcription of RNA regions with high secondary structure. It is stable over a broad range of reaction temperatures (42°C-65°C) and can achieve simultaneous genomic DNA (gDNA) removal and cDNA synthesis in one tube. This product generates the highest yields of cDNA, and is able to synthesize very long RNA transcripts up to 20 kb. The cDNA products are suitable for downstream PCR, qPCR and RNA-Sequencing applications.

User Guide

High thermostability: ThermoStable Reverse Tramscriptase is active up to 65°C.
Double advantage: Simultaneous gDNA removal and cDNA synthesis.
Higher yield: Synthesize very long RNA transcripts up to 20 kb.


Where can I find more information about Biotool?

Visit the manufacturer page at www.stratech.co.uk/biotool, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


Product Focus: Sepharose Resin for Protein Purification from Biotool

Ni-IDA Sepharose gel for His Tag Protein Purification

Biotool Ni-IDA Sepharose gel for His Tag Protein Purification uses sepharose CL4B as the substrate. An Ni metal ion is chelated onto the gel through the classical IDA ligand. Ni-IDA ligand has a stronger binding ability to His-tag in which affinity reaction every Ni2+ and Histone amino acid forms two coordinate bond.

Biotool Ni-IDA Sepharose gel for His Tag Protein Purification acquires binding capacity of 40 mg/ml at most, and with its nature of stability, simplicity, good repeatability, and convenience.

Biotool Ni-IDA Sepharose gel is an ideal tool for your demand of His-tag recombinant protein purification.

Brouchure

Simple Protocol

High Reliability

The recombinant protein is from E.coli cell lysate with a MW about 27KD


Where can I find more information about Biotool?

Visit the manufacturer page at www.stratech.co.uk/biotool, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


Product Focus: proNGF Rapid ELISA Kit – Mouse and Rat

The Biosensis proNGF RapidTM enzyme-linked immunosorbent assay (ELISA) Kit (Cat No.BEK-2236-1P-BIS) is a sandwich ELISA that allows the quantification of rodent full-length proNGF protein in less than 4 hours. This kit contains a recombinant mouse proNGF standard expressed in E.coli and consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-proNGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3’,5,5’-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proNGF present in samples and protein standards.

This ELISA kit is expected to detect rat proNGF due to high degree of homology (96%) with mouse proNGF based on amino acid sequence. In the absence of a true rat proNGF standard, results may be expressed as mouse proNGF equivalents!

This ELISA kit shows only 20% reactivity with the human form of proNGF and is therefore not suitable to quantify human proNGF. No cross-reactivity was observed with mature mouse NGF and full-length proBDNF. The antibodies used in this ELISA kit bind epitopes within the pro-domain (capture) and mature domain (detection) of the protein, thus this ELISA assay does not detect the pro-domain peptide.

The purpose of this kit is the quantitative determination of proNGF in cell culture supernatants and cell lysates. This kit has not been tested for other applications. Sufficient amount of proNGF standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required.

Kit ProtocolData Sheet

BEK-2236_Mouse proNGF ELISA Calibration Curve_web

This standard curve generated in our laboratories is for demonstration purposes only, but can be used as a guide to expected performance. A standard curve should be generated for each assay.


Where can I find more information about Biosensis?

Visit the manufacturer page at www.stratech.co.uk/biosensis, email info@stratech.co.uk or call +44 (0) 1638 782600

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


Product Focus: Quick-Fusion Cloning Kit

The Quick-Fusion Cloning Kit is designed for fast, direct cloning of DNA fragment into any vector at any site. By using this kit, researchers avoid the necessity for restriction enzyme digestionof PCR product and the selection of restriction enzyme site will be flexible. Additionally, the linearized vector and PCR product can be cyclized in only 30 minutes at 37℃. Compared to the conventional cloning method, a 15-20 base tract is added at the ends of PCR primers corresponding to the ends of the linearized vector.

Manual

Advantages

  • No ligase required
  • No PCR fragment cleaving required
  • Fast fusion, only 30 minutes
  • DNA fragment can be cloned into any vector at any site

Overview


Where can I find more information about Biotool?

Visit the manufacturer page at www.stratech.co.uk/biotool, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


Supplier Focus: Biotool – Hot Start High-Fidelity DNA Polymerase

Universe Hot Start High-Fidelity DNA polymerase

User GuideMSDS

Universe High-Fidelity Hot Start DNA polymerase is a superior enzyme in this category for robust PCR with extreme fidelity, featuring 53x error rate lower than Taq polymerase and extension rate as fast as 1 sec / kb*. Universe High-Fidelity Hot Start DNA polymerase possesses 5´→ 3´polymerase activity, 3´ → 5´exonuclease activity and will generate blunt-ended products. The polymerase is also capable of amplifying long fragments such as 20kb genomic DNA and 40kb λDNA. Our suggested applications include cloning, sequencing, genomic DNA amplification and high throughput PCR, etc.

Advantages

  • Robust: Maximal success with minimal optimization
  • Extreme Fidelity: 53x error rate lower than Taq
  • High Yield: Increased product yield using minimal enzyme
  • Versatile: Used for routine PCR, or with long, difficult templates

Materials Provided

Component B21101 100U (100 runs) B21102 250U (250 runs) B21103 500U (500 runs)
Universe High-Fidelity Hot Start DNA polymerase (1 U /μl) 100 μL 250 μL 500 μL
2 x Universe buffer 1.25 mL x 2 1.25 mL x 5 1.25 mL x 10
dNTP Mix (10mM each) 100 μL 250 μL 500 μL
10 x Loading buffer 1.25 mL 1.25 mL x 3 1.25 mL x 5

Universe Hot Start High-Fidelity 2x PCR Master Mix

Universe Hot Start High-Fidelity 2x PCR Master Mix

Biotool Universe Hot Start High-Fidelity 2x PCR Master Mix is a convenient premix version of Universe High-Fidelity Hot Start DNA Polymerase, which is a superior enzyme in this category for robust PCR with extreme fidelity, featuring 53x error rate lower than Taq polymerase and extension rate as fast as 1 sec / kb*. Universe High-Fidelity Hot Start DNA Polymerase possesses 5´→ 3´polymerase activity, 3´ → 5´exonuclease activity and will generate blunt-ended products. The polymerase is also capable of amplifying long fragments such as 20kb genomic DNA and 40kb λDNA. Our suggested applications include cloning, sequencing, genomic DNA amplification and high throughput PCR, etc.
* The extension rate varies with individual application.

Advantages

  • Robust: Maximal success with minimal optimization
  • Extreme Fidelity: 53x error rate lower than Taq
  • High Yield: Increased product yield using minimal enzyme
  • Versatile: Used for routine PCR, or with long, difficult templates
  • Convenient: Premix allows rapid reaction set-up

Materials Provided

Component B22101 (100 rxns) B22102 (250 rxns) B22103 (500 rxns)
Universe Hot Start High-Fidelity 2x PCR Master Mix 1.25mL x 2 1.25mL x 5 1.25mL x 10

Amplification Chart

Fig1: Amplification of genomic DNA templates of various lengths(0.6kB, 1.0kb, 2.6kb, 3.0kb, 4.0kb, 5.1kb, 6.2kb, 7.1kb, 8.5kb, 10.6kb, 17.8kb, 20.3kb, 21.4kb) using Universe High-Fidelity Hot Start DNA polymerase at extension rate of 15 seconds/kb.

Fig2: Amplification of λDNA and human genome DNA templates at various extension rate of 1sec/kb, 2 sec/kb, 5 sec/kb, 10 sec/kb, 15 sec/kb, 30 sec/kb, 60 sec/kb, respectively.


Where can I find more information about Biotool?

Visit the manufacturer page at www.stratech.co.uk/biotool, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


Product Focus: ECL Western Blotting Detection Substrate

The Biotool ECL Western Blotting Substrate was designed for the detection of horseradish peroxidase (HRP)-labelled proteins or nucleic acids. In the chemiluminescence western blotting detection, the sensitivity was not a limiting factor due to the use of luminous enhancer. Instead, the stability and reliability, such as avoiding non-specific bands or background bands or quenching, become the key elements for successful western blotting.

ECL Western Blotting Detection Substrate

Advantages

  1. Uses accurate luminous substrate to effectively reduce non-specific bands and background bands.
  2. Contains unique luminous enhancer to detect weak signals.
  3. Uses a special luminous recipe to laminate quickly and stably.
  4. Not interfere with proteins on the membrane. After exposure, the blot can be stripped and re-probed several times.

The properties of this kit include:

  1. Uses accurate luminous substrate to effectively reduce non-specific bands or background bands.
  2. Contains unique luminous enhancer to detect weak signals.
  3. Uses a special luminous recipe to flash quickly and stably.
  4. Does not destroy the proteins on the membrane. Through exposing and washing, other proteins can also be detected.

Where can I find more information about Biotool?

Visit the manufacturer page at www.stratech.co.uk/biotool, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


Supplier Focus: Biotool – qPCR Master Mixes

Which One Is Right for You ?

SYBR Green qPCR Master Mix   Probe qPCR Master Mix  
Specificity

Medium*

High

Sensitivity – Low Copies

Variable*

1-10 copies

Predesigned Assays    
Experimental Optimiation    
Gene Expression

Low Level of Quantitation

High Level of Quantitation

Illustration    
Applications
  • Dye-based quantitative PCR detection
  • Quantitative genotyping studies
  • Genomics-related applications
  • Dye-based quantitative PCR detection
  • Mutation Detection
  • Copy number variants
Higher Sensitivity    


SYBR Green qPCR Master Mix

User GuideMSDS

Biotool™ 2x SYBR Green qPCR master mix utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR Green I based quantitative PCR. The mix, prepared at 2x reaction concentration, can be directly used for robust and low-template quantitative PCR with high sensitivity, specificity and reliability.

Advantages

  • Accurate Detection: High specific and sensitive quantitation even for low copy targets or a wide range of template samples
  • High Stability: Hot-start enzyme activation and convenient room-temperature reaction setup makes it suitable for automation and high throughput detection
  • Robustness and Versatility: High-efficiency, compatible with both fast and standard qPCR cycling methods
  • Cost Effective: Offered at much discounted price compared to market price

Applications

  • Dye-based quantitative PCR detection
  • Nucleic acid amplification and expression profiling
  • Quantitative genotyping studies
  • Genomics-related applications

Components

Component Cat: B21202-BIT Cat: B21203-BIT Cat: B21204-BIT
Biotool™ 2x SYBR Green qPCR Master Mix 5 mL
(for 200 reactions with 50 μl/rxn
for 500 reactions with 20 μl/rxn
for 1000 reactions with 10 μl/rxn)
25 mL
(for 1000 reactions with 50 μl/rxn
for 2500 reactions with 20 μl/rxn
for 5000 reactions with 10 μl/rxn)
50 mL
(for 2000 reactions with 50 μl/rxn
for 5000 reactions with 20 μl/rxn
for 10000 reactions with 10 μl/rxn)
50x ROX Dye (low conc.) 200 μL 1000 μL 2000 μL
50x ROX Dye (high conc.) 200 μL 1000 μL 2000 μL
 Contains hot-start DNA Polymerase, dNTPs, Mg2+, and SYBR Green I dye

 qPCR Master Mix

User GuideMSDS

Biotool™ 2x probe qPCR master mix utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform fluorescence labeled probe based quantitative PCR (qPCR). The mix, prepared at 2x reaction concentration, can be directly used for robust and low-template quantitative PCR with high sensitivity, specificity and reliability.

Advantages

  • Accurate Detection: High specific and sensitive quantitation even for low copy targets or a wide range of template samples
  • High Stability: Hot-start enzyme activation and convenient room-temperature reaction setup makes it suitable for automation and high throughput detection
  • Robustness and Versatility: High-efficiency, compatible with both fast and standard qPCR cycling methods
  • Cost Effective: Offered at much discounted price compared to market price

Applications

  • Dye-based quantitative PCR detection
  • Nucleic acid amplification and expression profiling
  • Quantitative genotyping studies
  • Genomics-related applications

Components

Component Cat: B21301-BIT Cat: B21302-BIT Cat: B21303-BIT
Biotool™ 2x Probe qPCR Master Mixa[a] 1.25 ml (for 50 reactions with 50 μl/rxn) 5 ml (for 200 reactions with 50 μl/rxn) 25 ml (for 1000 reactions with 50 μl/rxn)
50x ROX Dye (low conc.) 50 μl 200 μl 1000 μl
50x ROX Dye (high conc.) 50 μl 200 μl 1000 μl
[a] Contains hot-start DNA Polymerase, dNTPs and Mg2+

Where can I find more information about Biotool?

Visit the manufacturer page at www.stratech.co.uk/biotool, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.