Product Focus: Thermophilic Polymerases for Maximum Flexibility

Jena Bioscience offers a broad range of optimized thermophilic polymerases. Make your selection from Taq polymerases for routine PCR applications, hot start polymerases for high specific amplifications or proofreading enzyme blends for high fidelity and long range PCR. All enzymes ensure reliable, high performance results and guarantee maximum success for their particular application.

Standard PCR – Product Selection Guide


Taq Pol – Thermostable DNA polymerase


Taq Pol / high yieldThermostable DNA polymerase

Taq Pol / high yield is recommended for use in routine PCR reactions. The buffer system is optimized for high efficiency and gives superior amplification results in a broad range of reaction conditions with most primer-template pairs. The buffer system facilitates the incorporation of labeled or modified nucleotides into DNA. Note that the ammonium based buffer contains detergents and may interfere with automated pipetting systems.
The enzyme replicates DNA at 72°C. It catalyzes the polymerization of nucleotides into duplex DNA in 5′→3′ direction in the presence of magnesium. It also possesses a 5′→3′ polymerization-dependent exo-nuclease replacement activity but lacks a 3′→5′ exonuclease activity.
Taq Pol – red / high yield buffer contains an inert red dye for easier handling and to facilitate the preparation of the master mix.


Hot Start PolHeat-activatable DNA polymerase for high specificity

Hot Start Pol provides improved specificity and sensitivity when amplifying low-copy-number targets in complex backgrounds or when prolonged room-temperature set up is required. The polymerase activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup.
The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5′→3′ direction in the presence of magnesium. It also possesses a 5′→3′ polymerization-dependent exonuclease replacement activity but lacks a 3′→5′ exonuclease activity.
Hot Start Pol requires no prolonged activation step. The polymerase inhibiting ligand is quickly released at the increased temperature of thermal cycling.
Hot Start Pol – red contains an inert red dye for easier handling and to facilitate the preparation of the master mix.


Rapid Pol - Thermostable DNA polymerase for rapid PCR

Rapid Pol is based on a specifically processed enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase.
The rapid polymerase is designed for Fast PCR, in which total reaction times are 20-70% shorter compared with conventional PCR assays. This can be achieved without sacrificing reaction performance or the requirement for specialized PCR consumables or thermocyclers.


High Fidelity PolDNA polymerase for high accuracy

High Fidelity Pol is based on a blend of Taq DNA polymerase and a proofreading enzyme specially designed for highly accurate and efficient amplification. It shows excellent results with extremely long (up to 30 kb), GC-rich or other difficult templates.
The enzyme blend includes a highly processive 5′→3′ DNA polymerase and possesses a 5′→3′ polymerization-dependent exonuclease replacement activity. Its inherent 3′→5′ exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase.
The enzyme is highly purified and free of bacterial DNA.


High Fidelity Hot Start PolHeat-activatable DNA polymerase for high accuracy and specificity

High Fidelity Hot Start Pol is based on a blend of Taq DNA polymerase and a proofreading enzyme specially designed for highly accurate and efficient amplification. The additional hot-start function provides improved specificity and sensitivity when amplifying low-copy-number targets in complex backgrounds or when prolonged room-temperature set up is required. The polymerase activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup.
The enzyme shows excellent results with extremely long (up to 30 kb), GC-rich or other difficult templates.
Activation step
High Fidelity Hot Start Pol requires no prolonged heating or denaturing step. The polymerase inhibiting antibodies are released at the increased temperature of the initial denaturation.


Pfu-X PolymeraseProofreading DNA polymerase for highest accuracy

Pfu-X Polymerase is the ideal choice for applications where the efficient amplification of DNA with highest fidelity is required.
The enzyme is a genetically engineered Pfu DNA polymerase, but showing a 2-fold higher accuracy and an increased processivity, resulting in shorter elongation times.
The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5′→3′ direction but does not possess a 5′→3′ exonuclease replacement activity. Its inherent 3′→5′ exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase. Pfu-X Polymerase-generated PCR fragments are blunt-ended.
The enzyme is highly purified and free of bacterial DNA.


Sequencing Pol - Taq Pol mutant for incorporation of ddNTPs

Sequencing Pol is a Taq polymerase mutant showing an enhanced efficiency for incorporation of ddNTPs. Its capability of incorporating ddNTPs and dNTPs at equal rates makes it the ideal choice for DNA cycle sequencing.
The enzyme replicates DNA at 74°C. It catalyzes the polymerization of nucleotides into duplex DNA in 5′→3′ direction in the presence of magnesium and has a 5′→3′ polymerisation-dependent exonuclease replacement activity only.


Where can I find more information about Jena Bioscience?

Visit the manufacturer page at www.stratech.co.uk/jena_bioscience, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


 

Product Focus: Shrimp Alkaline Phosphatase (SAP)

Shrimp Alkaline Phosphatase (SAP) is a high specific activity, heat-labile alkaline phosphatase  purified from a recombinant source and originally isolated from Pandalus borealis (arctic shrimp).  SAP is useful in many molecular biology applications such as the dephosphorylation of phosphorylated ends of DNA or RNA for subsequent use in cloning or end-labeling of probes. In cloning, dephosphorylation prevents relegation of linearized plasmid DNA. SAP may also be used to treat unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis.

Proven Performance – the Phosphatase benchmark

  • 100% heat-inactivated in 5 min at 65°C
  • Significantly improved storage stability at lower temperatures (see Fig. 1)
  • Very high specific activity (see Fig. 3)
  • Removes 5’-phosphates from DNA, RNA, dNTPs, and proteins
  • Purified from a recombinant source
  • May be added directly to restriction enzyme digests
  • No vector purification necessary
  • Requires no supplemental zinc or other additives for activity
  • Works direct in many different buffers
  • Easy treatment of unincorporated dNTPs in PCR products prior to DNA sequencing or SNP analysis

Shrimp Alkaline Phosphatase has approximately the same specific activity as Calf Intestinal Alkaline Phosphatase (CIAP), and like CIAP, is active in virtually all restriction enzyme reaction buffers. Unlike CIAP, Shrimp Alkaline Phosphatase is completely and irreversibly inactivated by heating reactions at 65°C for 15 min.

Shrimp Alkaline Phosphatase is particularly useful in preparing PCR products for applications involving sequencing, SNP analysis or labeling methods. Typically, excess dNTPs remaining after PCR interfere with subsequent enzymatic reactions involving DNA synthesis. SAP dephosphorylates all of the remaining dNTPs from the PCR mixture in one easy step.

Affymetrix is the world-wide provider of USB Shrimp Alkaline Phosphatase and we are pleased to be offering a recombinant version of our phosphatase benchmark.  Recombinant SAP eliminates the dependence on animal sourcing and offers the added benefits of increased storage stability and batch to batch consistency while providing exceptional enzymatic activity and 100% heat inactivation.


Where can I find more information about Affymetrix USB?

Visit the manufacturer page at www.stratech.co.uk/affymetrix, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


 

 

Product Focus: CytoGlow™ Cell-Based ELISA Kits

The CytoGlow™ Cell-Based ELISA utilizes an indirect ELISA format to allow for high-throughput screening on the effects of various drugs, metabolites, and treatment conditions on protein expression in whole cells.

Features

  • Detection of protein markers in adherent and suspension cells
  • Convenient and ready-to-use
  • Sample preparation not necessary
  • Minimum of 5,000 cells required per well
  • High-sensitivity, low background with exceptional signal-to-noise ratio
  • 450 nm colorimetric detection
  • Can be used with human mouse and rat targets in their respective cell lines
 

Click Image to Enlarge


Where can I find more information about Assay Biotech?

Visit the manufacturer page at www.stratech.co.uk/assay_biotech, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


 

Product Focus: OmniKine™ Traditional Sandwich ELISA Kits from Assay Biotech

The OmniKine™ Traditional Sandwich ELISA is Assay Biotech’s most popular ELISA kit, providing a standard sandwich ELISA platform for the detection of cytokines, growth factors, and respective receptors in a variety of biological samples including cell lysates, supernatants, sera and plasma. Through these OmniKine™ ELISAs, offer by Assay Biotech provide excellent sensitivity and product usability.

Features

  • Detection of proteins from pg/ml to ng/ml concentrations
  • Convenient and ready-to-use
  • High-sensitivity, low background with exceptional signal-to-noise ratio
  • 450 nm colorimetric detection
  • Human, mouse and rat targets
  • For use with cell lysates, supernatants, sera and plasma

Click to enlarge


Where can I find more information about Assay Biotech?

Visit the manufacturer page at www.stratech.co.uk/assay_biotech, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


 

Product Focus: VeriQuest Probe qPCR Master Mix

VeriQuest Probe qPCR Master Mix is supplied as a 2X pre-mixed formulation containing both ROX and UDG in an optimized buffer for quality results in real-time quantitative PCR assays. The proprietary reaction buffer with optimum MgCl2 concentration is specifically designed for robust probe hybridization and efficient cleavage of TaqMan probes. Simply add DNA template, primers, probe(s), and water and the reactions are ready for cycling. The master mix contains a chemically-modified Taq DNA Polymerase, MgCl2, ultrapure nucleotides with an optimized dUTP:dTTP ratio, Uracil-DNA Glycosylase (UDG or UNG), and ROX Passive Reference Dye in a proprietary reaction buffer.

Features:

  • One-tube master mix with ROX Reference Dye included
  • Similar protocols, same probes, and same primers for standard mode amplification
  • Consistency over a broad dynamic range: 9 orders of magnitude linear detection range with a correlation coefficient = 0.999
  • Exceptional performance with challenging GC-rich regions
  • Detect 2 copies of target from genomic DNA and differentiate less than a 2-fold differences
  • Contains dUTP and Uracil-DNA Glycosylase (UDG or UNG) for optional carryover contamination prevention.
  • Hot start PCR for room temperature reaction setup
  • Stable at room temperature for 72 hours in a preassembled reaction
  • Validated for use with a variety of 5’ reporter fluorophores and 3’ quenchers, as well as TaqMan MGB containing probes

Simplify the process with a single tube master mix containing UDG and ROX passive reference dye

Catalogue No. Description Unit Size
75650_40_RXN-AFM
VeriQuest Probe qPCR Master Mix (2X)
1 ml for 40 reactions (50 µl)
75650 200 RXN-AFM
VeriQuest Probe qPCR Master Mix (2X)
5 ml for 200 reactions (50 µl)
75650 400 RXN-AFM
VeriQuest Probe qPCR Master Mix (2X)
2 x 5 ml for 400 reactions (50 µl)
75650 1000 RXN-AFM
VeriQuest Probe qPCR Master Mix (2X)
5 x 5 ml for 1,000 reactions (50 µl)
75650 2000 RXN-AFM
VeriQuest Probe qPCR Master Mix (2X)
10 x 5 ml for 2,000 reactions (50 µl)

Literature


Where can I find more information about Affymetrix USB?

Visit the manufacturer page at www.stratech.co.uk/affymetrix, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


Product Focus: Ultra Linked Protein A Resin

Sino Biological Ultra-linked rProtein A resin is a high capacity, high throughput and low leaching medium for purification of monoclonal or polyclonal antibodies. The resin is stable, durable, cost-effective, and suitable for both pilot and large scale purification.

Advantages

  • High Binding Capacity
  • High Throughput
  • Low Protein A Leaching
  • CIP Stability

High Binding Capacity

As a result of the special coupling methods, Sino Biological Ultra-linked rProtein A resin provides a high binding capacity, with a dynamic binding capacity over 30 mg for human IgG.

Antibody loaded (mg) recovery %
30 99.0
35 96.6
40 87.5

Antibody recovery in eluant when specific anmount was applied on 1 ml column
Binding buffer: 50mM Tris, 100mM NaCl, pH8.0
Elution buffer: 100mM Gly, 50mM NaCl, pH3.0
Column bed volume: 1 ml; Flow rate: 0.2 ml/min


High Throughput

Sino Biological Ultra-linked rProtein A resin was produced by immobilizing recombinant protein A to 4% cross-linked agarose matrix. The matrix is highly cross-linked, physically and chemically stable, and particularly suitable for process scale applications where starting material volumes are large and flow rates are high. The maximum flow rate is 250 cm/h.

Pressure / flow rate curve (1.3*20cm,CV=26.5ml,water)


Low Protein A Leaching

The ligand protein A has been coupled to the matrix through a stable covalent bond . And the leached protein A ligand is far below 100 ppm.


CIP Stability

The column was loaded with antibody at 30mg/ml for 5min. The antibody recovery remains over 96%, after 30 cycles of CIP.
One CIP cycle includes the following washing steps: Washing: 0.05M NaOH, 1 M NaCl, 0.2ml/min, 6 column volumes (CV), Balancing: 5 CV, Eluting: 5 CV


Where can I find more information about Sino Biological?

Visit the manufacturer page at www.stratech.co.uk/sino_biological, email info@stratech.co.uk or call +44 (0) 1638 782600

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


Product Focus: PowerFluor™ Immunofluorescence Mouse Detection kit

PowerFluor™immunofluorescence mouse detection kit is designed to localize mouse primary antibodies on human, goat and rabbit tissue in immunofluorescence applications. PowerFluor™ uses biotin-free technology and it completely eliminates the potential background due to endogenous biotin activity on biotin-rich tissues when use Biotin-Strepavidin-Fluorphore technology. The MaxFluor™ Dyes range exhibit bright fluorescence intensity and photostability. Also they remains highly fluorescent over a broad pH range (pH 4-9). This make them an ideal choice for researcher’s immunofluorescence applications. PowerFluor™ uses a proprietary fluorescence signal amplification technology so the researchers may expect the best signal-to-noise ratio using the PowerFluor™ImmunoFluorescence Detection Kit when compare with the direct labeling technology or other indirect methods, such as Biotin-Streptavidin method.

Key Benefits:

  • Bright and stable fluorescence
  • Biotin-free signal amplification
  • Super clean background
  • Significant increase in fluorescence signal
  • 3-4 times more sensitive and cleaner background than direct method
  • No biotin blocking steps and completely elimination of biotin background
  • Best signal-to-noise ratio

For immunofluorescence detecting mouse primary antibodies on human, rabbit and goat specimen

Cat No. Description Size

Dye

Primary Antibody

Tissue

Datasheet

PF11-M-MAX PowerFluor™488 Immunofluorescence Mouse Detection Kit

18ml

MaxFluor 488

Mouse

Hu, Rb, Gt

PDF

PF11-S-MAX PowerFluor™488 Immunofluorescence Mouse Detection Kit

6ml

MaxFluor 488

Mouse

Hu, Rb, Gt

PDF

PF12-M-MAX PowerFluor™550 Immunofluorescence Mouse Detection Kit

18ml

MaxFluor 550

Mouse

Hu, Rb, Gt

PDF

PF12-S-MAX PowerFluor™550 Immunofluorescence Mouse Detection Kit

6ml

MaxFluor 550

Mouse

Hu, Rb, Gt

PDF

PF13-M-MAX PowerFluor™594 Immunofluorescence Mouse Detection Kit

18ml

MaxFluor 594

Mouse

Hu, Rb, Gt

PDF

PF13-S-MAX PowerFluor™594 Immunofluorescence Mouse Detection Kit

6ml

MaxFluor 594

Mouse

Hu, Rb, Gt

PDF

PF14-M-MAX PowerFluor™650 Immunofluorescence Mouse Detection Kit

18ml

MaxFluor 650

Mouse

Hu, Rb, Gt

PDF

PF14-S-MAX PowerFluor™650 Immunofluorescence Mouse Detection Kit

6ml

MaxFluor 650

Mouse

Hu, Rb, Gt

PDF


Where can I find more information about MaxVision?

Visit the manufacturer page at www.stratech.co.uk/maxvision, email info@stratech.co.uk or call  +44 (0) 1638 782600

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


 

Product Focus: VeriKine-HS™ Mouse Interferon Beta Serum ELISA Kit

The versatility of mouse models lends their use in a number of disease and autoimmunity studies. Interferon β plays a pivotal role in the protective response to many infections and diseases due to its antiviral and immune-modulatory activities, and is an important early product of TLR/RLR stimulation.

PBL’s 96-well ELISA kit will equip you with the most sensitive and consistent tool for accurately measuring Mouse IFN-β in serum and plasma matrices. Purified, recombinant mouse IFN-β expressed in mammalian cells is provided as the standard. The sensitivity provided by this kit, to less than 1 pg/ml in serum and plasma, will propel your mouse studies and help uncover insights into the pathology of diseases.

Key Benefits & Features

  • The world’s most sensitive Mouse IFN Beta ELISA
  • Quantifies low level mouse IFN-β in serum, plasma and tissue culture media with an assay range of 0.94-60 pg/ml
  • Reliable serum and plasma spike recoveries of >90%
  • Provides reproducible results with inter- & intra-assay CVs of <10%
  • Total assay time of ~2 hours

Related technical information


Catalogue No. Description Pack Size
42410-1 VeriKine-HS™ Mouse Interferon Beta Serum ELISA Kit

  • Compatibility:Serum, Plasma, Tissue Culture Media
  • Assay Range:0.94-60 pg/ml
1 x 96-well plate
4210-2 VeriKine-HS™ Mouse Interferon Beta Serum ELISA Kit

  • Compatibility:Serum, Plasma, Tissue Culture Media
  • Assay Range:0.94-60 pg/ml
5 x 96-well plate

Product Specific Information


References

  1. Staehelin, T., Stahli, C., Hobbs, D.S., and Peskta, S. (1981) “A Rapid Quantitative Assay of High Sensitivity for Human Leukocyte Interferon with Monoclonal Antibodies,” Methods in Enzymology, Vol. 79 (S. Peskta, ed.), Academic Press, New York, 589-595.
  2. Balachandran, S,, Thomas, E., and Barber, G. (2004) “A FADD-dependent innate immune mechanism in mammalian cells,” Nature, Vol 432, 401-405.

Where can I find more information about PBL Assay Science?

Visit the manufacturer page at www.stratech.co.uk/pbl_assay_science, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


 

Product Focus: Prep2Seq™ DNA Library Prep Kit

for Illumina platform

DNA library prep is critical to the precision and accuracy of NGS. The Prep2Seq DNA Library Prep Kit comes with all of the reagents necessary for end repair, A-tailing, and ligation, and can be completed in less than two hours.

Generates high quality next generation sequencing (NGS) libraries suitable for sequencing on Illumina platforms in less time with Prep2Seq DNA Library Prep Kit.

Affymetrix USB’s kit is fast, efficient, and optimized. It efficiently converts 3′ and 5′ overhangs into 3′ A-overhang ends for ligation to T-tailed DNA adapters and reduces the potential for bias with our superior A-tailing and ligation steps. This kit offers significant improvement in the quality of sequence-ready libraries by eliminating chimeras and reducing side reaction fragments.

The Prep2Seq Library Prep Kit combines end repair and A-tailing steps, decreasing the total number of steps to achieve quality NGS library preparation. Only one clean up step is required to attain peak performance on the Illumina sequencing platform and only 100 ng of input is needed.

The Prep2Seq DNA Library Prep Kit offers the highest adapter ligation efficiency available. By combining end repair and A-tailing, the Prep2Seq kit is able to reduce total NGS library prep time to 110 minutes and limit bead cleanup to a single step.

Rigorous comparison studies to competitor kits show that the Prep2Seq DNA Library Prep Kit generates impressive sequencing data.

The Prep2Seq DNA Library Prep Kit ensures efficiency of ligation to T-tailed adapters by optimizing the A-tailing step. This enzymology focus on A-tailing ensures that end bias is reduced, and the formation of blunt- and G-tailed ends is minimized. Most enzymes used for A-Tailing are inefficient and result in a large amount of blunt-end DNA that can ligate as concatemers, forming chimeric false joints and compromising sequencing data. Effective A-tailing is crucial in preparing efficient DNA adapter libraries that are ready for sequencing.

Time savings with a more efficient method for NGS sample prep

  • Improved A-tailing reaction with proprietary enzymes, cloning know-how, and optimized buffers.
  • By combining the end repair and A-tailing steps, only one cleanup step is needed compared to the two or three steps in most other kits (saving up to two and a half hours in the lab).

Full kit offering from Affymetrix USB:

  • Single kit that includes all reagents for sample prep in a slimmed down protocol.
  • 24 bar code adapter sequences compatible with Illumina technology will also be available for purchase: Prep2Seq Multiplex Oligo Adapters for Illumina
    • Index Adapter Set I – includes adapter sequences 1-12 (79800 1 KT)
    • Index Adapter Set II – includes adapter sequences 13-27 (79800 2 KT)
Kit Components
This kit is designed for input sheared genomic DNA amounts from 100 ng to 1 µg and consists of 3 tubes: Prep2Seq NGS Enzyme Mix, Prep2Seq NGS 2X Buffer, and Prep2Seq NGS High Concentration Ligase. A streamlined workflow, straightforward protocol, and high performing kit ensures quality library prep for your Illumina sequencing runs.

Per 10 reactions:

  • Prep2Seq NGS Enzyme Mix, 40 µl
  • Prep2Seq NGS 2X Buffer, 250 µl
  • Prep2Seq NGS High Concentration Ligase, 40 µl

Complete kit with the buffers and enzymes needed for high performing NGS libraries

Catalogue No. Description Unit Size
79900 10 RXN
Prep2Seq DNA Library Prep Kit
10 reactions
79900 20 RXN
Prep2Seq DNA Library Prep Kit
20 reactions

Technical Documents


Where can I find more information about Affymetrix USB?

Visit the manufacturer page at www.stratech.co.uk/affymetrix, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


Product Focus: VeriKine™ Human Interferon Alpha/Beta Receptor 2 ELISA Kit

The Type I interferon receptor is a heterodimer consisting of IFNAR1 and IFNAR2 1,2,. The IFNAR2 subunit provides most of the binding affinity for IFN and IFNAR1 is required for optimum signaling. IFNAR2 has two membrane forms and is also found soluble in blood and urine 3,4,5. IFNAR2a is the soluble form and can be generated either by alternative splicing or proteolytic cleavage. IFNAR2b is membrane-localized but acts as a decoy due to its lack of docking sites for the signal transduction kinases. IFNAR2c is the productive membrane-localized form involved in IFN signaling.

Elevated levels of soluble IFNAR2a have been demonstrated in various cancers 6,7,8 and autoimmune diseases 9,10 and membrane levels of IFNAR2b/c have been shown to be regulated by IFN signaling in certain cells11. Otherdisease states or ways of modulating the IFN receptor on cells have not been examined.

The PBL Assay Science VeriKine™ Human Interferon Alpha/Beta Receptor 2 ELISA is intended to detect soluble IFNAR2a in plasma/sera and other biological matrices. It also may be used to detect the soluble IFNAR2a in tissue culture media and the IFNAR2b and IFNAR2c in cell lysates.

The VeriKine™ Human Interferon Alpha/Beta Receptor 2 ELISA Kit provides:

  • High sensitivity and excellent specificity for detection of Human IFNAR2 to < 5 pg/ml
  • Conserve high value specimens with low sample requirements (1:100 dilutions of Normal Human Serum and Plasma)
  • Compatible with serum, plasma, cell lysate and cell culture supernatant
  • Able to measure the membrane-bound form of IFNAR2 in cell lysates and hence better quantify receptor density on cells compared to FACS
  • No significant cross-reactivity between Human IFNAR1 and analogues

Catalog No. 41385-1 VeriKine™ Human Interferon Alpha/Beta Receptor 2 ELISA Kit

  • Compatibility:Serum, Plasma, Cell Lysate, Cell Culture Supernatant
  • Assay Range:3.125 – 200 pg/ml

Further Information

Product Information

Related technical information


References

  1. Uzé G, Schreiber G, Piehler J, Pellegrini S. The receptor of the type I interferon family.Curr Top Microbiol Immunol.2007;316:71-95.
  2. Krause CD, Pestka S. Historical developments in the research of interferon receptors.Cytokine Growth Factor Rev.2007 Oct-Dec;18(5-6):473-82.
  3. Novick D, Cohen B, Tal N, Rubinstein M. Soluble andmembrane-anchored forms of the human IFN-alpha/betareceptor.J Leukoc Biol.1995 May;57(5):712-8.
  4. de Weerd NA, Samarajiwa SA, Hertzog PJ. Type Iinterferon receptors: biochemistry and biological functions.J Biol Chem.2007 Jul 13;282(28):20053-7
  5. Gilli F. Role of differential expression of interferon receptor isoforms on the response of multiple sclerosis patients totherapy with interferon beta.J Interferon Cytokine Res.2010 Oct;30(10):733-41.
  6. Tanaka S, Hattori N, Ishikawa N, Horimasu Y, Deguchi N,Takano A, Tomoda Y, Yoshioka K, Fujitaka K, Arihiro K,Okada M, Yokoyama A, Kohno N. Interferon (alpha, beta and omega) receptor 2 is a prognostic biomarker for lung cancer. Pathobiology. 2012;79(1):24-33.
  7. Ambrus JL Sr, Dembinski W, Ambrus JL Jr, Sykes DE,Akhter S, Kulaylat MN, Islam A, Chadha KC.Freeinterferon-alpha/beta receptors in the circulation ofpatients with adenocarcinoma.Cancer.2003 Dec 15;98(12):2730-3.
  8. Novick D, Cohen B, Rubinstein M. Soluble interferon-alpha receptor molecules are present in body fluids.FEBSLett.1992 Dec 21;314(3):445-8.
  9. Ambrus JL, Ambrus JL Jr, Chadha S, Novick D, Rubinstein M, Gopalakrishnan B, Bernstein Z, Priore RL, Chadha KC. Mechanism(S) of interferon inhibitory activity in blood from patients with AIDS and patients with lupus erythematosus with vasculitis.Res Commun Mol PatholPharmacol.1997 Jun;96(3):255-65.
  10. Chadha K, Weinstock-Guttman B, Zivadinov R, Bhasi K,Muhitch J, Feichter J, Tamaño-Blanco M, Abdelrahman N,Ambrus J Sr, Munschauer F, Ramanathan M. Interferoninhibitory activity in patients with multiple sclerosis.ArchNeurol.2006 Nov;63(11):1579-84.
  11. Marijanovic Z, Ragimbeau J, van der Heyden J, Uzé G,Pellegrini S. Comparable potency of IFN alpha2 and IFNbeta on immediate JAK/STAT activation but differential down-regulation of IFNAR2.Biochem J.2007 Oct1;407(1):141-51.

Where can I find more information about PBL Assay Science?

Visit the manufacturer page at www.stratech.co.uk/pbl_assay_science, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.