MicroRNAs (miRNAs) are short endogenous, non-coding RNAs that modulate gene expression through binding to messenger RNA. They may serve as cancer biomarkers, and various methods for their profiling and detection were reported.
Jena Bioscience’s approach relies on enzymatic attachment of a single “Marker”-pCp-nucleotide (pCp: Cytidine-3′,5′-bisphosphate) to the 3′-terminus of miRNA (Fig. 1). Common markers are biotin and fluorescent dyes, and such labeled miRNAs can be isolated via streptavidin or directly applied to microarray expression profiling, respectively.
Figure 1: A “Marker”-pCp is attached to the 3′-end of MicroRNA with T4 RNA ligase resulting in biotin- or fluorescence-labeled miRNA for further analysis.
- Simple direct labeling of sub-µg quantities of total RNA
- No need for amplification or size fractionation
- Little sequence bias and low detection limits
- Direct miRNA measurement in complex samples
- Yin et al. (2008) Profiling microRNA expression with microarrays. Trends in Biotechnology 26(2):70.
- Wang et al. (2007) Direct and sensitive miRNA profiling from low-input total RNA. RNA 13:151
Where can I find more information about Jena Bioscience?
Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.