NEW: hAstroPro Human Astrocyte+Astroglial-Neurons Co-Culturing Kits

Neuromics now provides ArunA Biomedical’s  first of  their kind human astrocyte-glial or astrocyte-glial-neuron co-culturing kits to meet your exacting specifications. Whether you require a pure astro-glial or mixed culture, we are proud to be the first offering you the right solution. Options:

  1. hAstroPro™ Human Astrocyte Cells are derived from human pluripotent stem cell line h9 (NIH registry number 0062) and are the first highly characterized hESC derived CNS support cell type commercially available. hAstroPro™ cells are derived using a chemically defined method that enables shortened differentiation and scaled uniform production.
  2. hAstroPro™+NeuroNet™ Pure Human Neurons are a highly pure population of hPSC derived neurons developed with versatility in mind. As a standalone model for studies of cellular and molecular neurobiology, drug discovery and neurotoxicity, NeuroNet™ Human Neurons enable rapid response in a relevant in vitro culture model system and have been strategically designed for co-planer cellular model studies.

Images: Astrocyte-Glial-Neuron Co-Culture Stained with MAP-2 (Green) and DAPI (Blue).


hAstroPro Human Astrocyte Kit

Cell Type:  Astrocytes
Part Number:  hAP7027
Species:  human
Source:  derived from h9 hESC (NIH registry number 0062)
Morphology:  glial
Quantity:  ~1×106cells per vial
Shipping method:  dry ice
Storage:  liquid nitrogen
Growth Properties:  adherent
Medium:  AB2™ Basal Neural Medium with ANS™ Neural Medium Supplement plus additional unsupplied reagents.
Purity as determinded by qPCR: S100β, CD44, BDNF, GFAP.


NeuroNet Pure Human Neurons

Quantity:  ~1×106 cells per vial
Shipping method:  dry ice
Storage:  liquid nitrogen
Growth Properties:  adherent, non-proliferative
Medium:  AB2™ Basal Neural Medium with ANS™ Neural Medium Supplement plus LIF (10 mg/mL)
Phenotyping:  Cells are >90% β III tubulin positive and >60% MAP2 positive.

DNA fingerprint:  Results are consistent with the presence of a single cell line with no evidence of culture cross contamination. The loci match the DNA fingerprint pattern for the H9 (NIH designation, WA09) hESC line as published in http://stemcells.nih.gov/research/nihresearch/scunit/.

Pathogen testing:   This lot was derived from the H9 hESC line that has been tested for Hepatitis B, Hepatitis C, HIV-1, HIV-2, HTLV-I/II, HSV1, HSV2, EBV, and CMV.  The H9 cell line has been tested and shown to be negative (performed by GIVF Laboratories).  Cells are tested for sterility and mycoplasma contamination.


Where can I find more information about Neuromics?

Visit the manufacturer page at www.stratech.co.uk/neuromics, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe.

Please contact us to find out which product ranges we can supply in your country.


 

Product Focus: Annexin V-FITC Kit

Benefits

  • Detects apoptosis earlier in the process than DNA-based assays (TUNEL).
  • Rapid labeling of cells. Cell staining takes only 10 minutes.
  • No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
  • PI is included with the kit to differentiate apoptotic cells from viable and necrotic cells.

Introduction

Annexin V-FITC kit provides a rapid and convenient assay for apoptosis.Annexin V-FITC kit detects the externalization of phosphatidylserine in apoptotic cells using recombinant annexin V conjugated to green-fluorescent fluorescein isothiocyante (FITC) dye and necrotic cells using propidium iodide (PI) by flow cytometry and fluorescence microscope. The AnnexinV-FITC kit uses annexin V conjugated with FITC to label phosphatidylserine sites on the membrane surface. The kit also includes propidium iodide (PI) stains necrotic cells with red fluorescence. After treatment with both probes, apoptotic cells show green fluorescence, dead cells show red and green fluorescence, and live cells show little or no fluorescence.

Catalogue B32115 (50 rxns) B32117 (100 rxns)
Annexin V-FITC 250 μl 250 μl×2
PI Staining Solution 250 μl 250 μl×2
1 × Binding Buffer 25 ml 25 ml×2

Storage

Store in refrigerator (2–8° C) and protect from light.


Experimental Protocol

1. Induce apoptosis in cells using the desired method. Prepare a negative control by incubating cells in the absence of inducing agent.
2. Harvest the cells after the incubation period and wash in cold phosphate-buffered saline (PBS).
3. Re-centrifuge the washed cells, discard the supernatant, and resuspend the cells in 100 μl 1 × Binding Buffer.
4. Add 5 μL Annexin V -FITC and 5 μL PI Staining Solution to each 100 μL of cell suspension.
5. Incubate the cells at room temperature for 15 minutes.
6. After the incubation period, add 400 μL of 1 × Binding Buffer, mix gently and keep the samples on ice.
7. As soon as possible, analyze the stained cells by flow cytometry, measuring the fluorescence emission at 530 nm (e.g., FL1) and >575 nm (e.g., FL3). The population should separate into three groups: live cells will show only a low level of fluorescence, apoptotic cells show green fluorescence and dead cells show both red and green fluorescence.Confirm the flow cytometry results by viewing the cells under a fluorescence microscope, using filters appropriate for fluorescein (FITC) and rhodamine (TRITC) or Texas RedR dye.

Biotool™ Annexin V-FITC Apoptosis Detection Kit Protocol


Where can I find more information about Biotool?

Visit the manufacturer page at www.stratech.co.uk/biotool, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


 

Product Focus: RNA in vivo transfection reagent

work well for siRNA, miRNA, guide RNA and long ncRNAs


Description

Biotool RNA in vivo Transfection Reagent is a nanoscale polymer based reagent, successfully used to in vivo transfer siRNA, miRNA (mimic and inhibitor), guide RNA or long ncRNAs for research purposes such as gene functional studies or RNAi in vivo studies. This reagent interacts with nucleic acids and moves together into cells via endocytosis. In addition to efficiently transporting nucleic acids into the cells, the formula is also able to lead to the protection of the nucleic acids from degradation in vivo. Biotool RNA in vivo Transfection Reagent has been proven to be able to perform the maximum delivery of siRNA, miRNA (mimic and inhibitor), guide RNA or long ncRNAs into different organs in the body. No significant pro-inflammatory responses have been detected using this reagent.

Biotool RNA in vivo Transfection Reagent performs high transfection efficiency in vivo.

Biotool RNA in vivo Transfection Reagent test: Systemic administration (i.v.) of Biotool RNA in vivo Transfection Reagent conjugated with siRNA (siRNA/mouse: 1mg/kg, 2mg/kg and 3mg/kg) targeting coagulation factor VII (FVII) gene mRNA or non-silencing control siRNA (3mg/kg) following our protocol. Liver was collected and total RNA isolated 48 hours after tail vein injection. Samples were analyzed by qRT-PCR for FVII mRNA expression levels. β-Actin RNA levels were used to normalize the expression data. Data are means +/- SD (n=6).

No detectable inflammatory response.

Serum concentration of IL12/IL23 and IL6 following intravenous nucleic acid delivery using Biotool RNA in vivo Transfection Reagent or saline (negative control) 24h after injection respectively. The positive control is an IP injection of 50μg inactivated E.coli (LPS). 40ug siRNA were delivered with or without Biotool RNA in vivo Transfection Reagent.

Products

B45212     20 injections (0.5 ml)
B45215     100 injections (2.5 ml)
B45218     1000 injections (25 ml)


Flow Chart


 Troubleshooting

 

Problem Possible Reason Suggested Improvement
Precipitate formation in transfection mixture RNA not dissolved in distilled H2O (e.g. Tris-EDTA) Prepare fresh culture.
Excessive RNA concentration in transfection mixture Ensure RNA concentration in transfection mixture ≤ 0.5 µg/µL before injection
Transfection mixture froze or was stored at low temperature for a long period of time Freshly prepare transfection mixture and use immediately
Low transfection efficiency Low administration dose Scale up administration dose in the case of exclusion of animal lethality
Inflammation response or lethality of animal Injection manipulation error Inject the transfection mixture at correct site by correct method
Excessive RNA amount Scale down RNA amount and adjust the volume of transfection reagent accordingly
Endotoxin in transfection mixture Prepare endotoxin-free RNA
Pass transfection mixture through a 0.22 µM sterile filter before injection

 


 User Guide

Biotool RNA in vivo Transfection Reagent.pdf


Where can I find more information about Biotool?

Visit the manufacturer page at www.stratech.co.uk/biotool, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


 

Product Focus: IDH1 R132H detection: Clone H09 is the gold standard.

In histological studies of human IDH1 R132H in formalin-fixed paraffin-embedded brain tissue sections antibody clone H09 displays a strong and specific reaction at a 1:40 dilution. In contrast, at a 1:40 dilution antibody clone HMab-1 shows a very faint staining which remains weak even at lower dilutions of 1:20 and 1:10.

The exclusive production of antibody clone H09 by dianova together with its widespread commercial availability since 2010 has changed routine neuropathology: It provides an unrevaled accuracy to the identification of infiltrating gliomas and secondary gliomas.

At present many companies non-exclusivly offer a different clone, HMab-1. By comparative IHC analysis we demonstrate that clone H09 displays an unchallenged staining quality at considerably higher dilution.

H09-HMab-FigFig.: IHC of human IDH1 R132H in formalin-fixed paraffin-embedded brain tissue sections: The IDH1 R132H mutation specific monoclonal antibody clone H09 displays a strong and specific reaction at a 1:40 dilution. In contrast, at a 1:40 dilution antibody clone HMab-1 shows a very faint staining which remains weak even at lower dilutions of 1:20 and 1:10. All sections were stained by an indirect alkaline phosphatase method according to standard procedures with antigen retrieval by high-temperature heating in citrate buffer (pH 6.0) and counterstaining with Hematoxylin.


Where can I find more information about Dianova?

Visit the manufacturer page at www.stratech.co.uk/dianova, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


Product Focus: Anti-Murine CD3 – Clone HH3E for standard FFPE tissues

Antibody clone HH3E (#DIA-303) has been specifically validated for the detection of murine CD3 in formalin-fixed paraffin-embedded tissue sections (mouse FFPE). CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as T cell marker. During T cell maturation the expression of CD3 migrates from the cytoplasm of pro-thymocytes to the cell-membrane of thymocytes.

The specific appearance at all stages of T cell development make CD3 an ideal marker for normal T cells and T cell neoplasms (lymphomas, leukemias). Moreover, CD3 is a usefull immunohistochemical marker for T cells in tissue sections.

In a clinical setting in humans, CD3 serves as an important T cell marker for the classification of malignant lymphomas and leukemias. An anti-CD3 epsilon antibody (Okt3) has been clinically approved for the induction of immunosuppression in organ transplantation. In animal studies anti-CD3 antibodies can induce tolerance to allografts.

Immunhistochemistry of mouse CD3e (TCRE) in standard-FFPE tissue sections.

(All sections were stained by an indirect alkaline phosphatase method according to standard procedures and counterstaining with Hämatoxylin. Pictures courtesy of Prof.H.Stein, Pathodiagnostik Berlin, Berlin, Germany)


Where can I find more information about Dianova?

Visit the manufacturer page at www.stratech.co.uk/dianova, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


 

SynVivo Tumor Assay Featured on the Cover of J Controlled Release

SynVivo have announced that the editors of the Journal of Controlled Release have selected a new paper from SynVivo CTO, Prabhakar Pandian, Ph.D, and his colleagues as the cover of this prestigious journal publication. The paper describes the team’s work to reproduce a realistic and dynamic tumor microenvironment and it’s use in screening drug delivery systems. The studies were performed using the SynVivo co-culture microfluidic cell-based assay device whereby they created an in vitro tumor microenvironment encompassing circulatory flow in the vessels, transport across the leaky vasculature near the tumor region and real-time visualization of drug deliver to 3D culture of tumor cells.

Synthetic Tumor Networks for Screening Drug Delivery Systems. B. Prabhakarpandian, MC Shen, J. Nichols, C. Garson, I. Mills, M. Matar, J. Fewell, K. Pant. J Control Release., 2015, 201, 49-55. doi:10.1016/j.jconrel.2015.01.018

 


Where can I find more information about SynVivo?

Visit the manufacturer page at www.stratech.co.uk/synvivo, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


 

Application Examples of SynVivo Technology in Action

SynVivo products are a physiological, cell-based microchip platform that provides a morphologically and biologically realistic microenvironment allowing real-time study of cellular behavior, drug delivery and drug discovery.

SynVivo’s patented technology for recreating microvasculature scale, shape, fluidics, and cellular constructs provides the most realistic in vitro testing platform for drug discovery, delivery, efficacy, and toxicity assays.

The examples below are specific applications of SynVivo’s technology to set examples where this system can bridge the gap to in vivo.


 Migration Assays

Imagine an assay which is repeatable, simple to use, provides real-time live cell imaging and gives data which matches in vivo observations. SynVivo has overcome the limitations of Transwell® assays to provide an entirely new system for studying migration in a realistic and dynamic environment.

Read more: Migration Assays


Nanopolymer Delivery for Gene Therapy

A nanopolymer used for drug delivery was complexed with GFP DNA and perfused on tumor cell (HeLa) cultured networks. GFP transfection, efficiencies and distribution was quantified at 24 hours post injection.

Read more: Nanopolymer Delivery for Gene Therapy


Targeted Drug Delivery

A confluent layer of endothelial cells were activated with cytokines. Anti-ICAM-1 coated particles were used for targeting (red dots) and IgG particles served as controls (green dots). Data was obtained at 4 and 24 hours post treatment.

Read more: Targeted Drug Delivery


Drug Particle Adhesion

P-selectin, an inflammatory molecule was coated on the walls of SynVivo. Particles coated with the antibody to P-selectin were injected into SynVivo and their differential binding pattern was observed.

Read more: Drug Particle Adhesion


Drug Perfusion

SynVivo were injected with trypan blue and the perfusion of the dye was observed in real-time.

Read more: Drug Perfusion


Where can I find more information about SynVivo?

Visit the manufacturer page at www.stratech.co.uk/synvivo, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


 

Make your own FPLC columns!

Simply pack your own FPLC columns with any of Jena Bioscience’s bulk chromatography agaroses suitable for FPLC devices (see Table below). Their empty single-use FPLC columns and Luer Lock Male & Female connectors

    • are suitable for common FPLC instruments such as ÄKTA™ design instruments
    • are available at four different sizes (8 ml, 30 ml, 45 ml and 80 ml bed volume)

Figure 1: A) FPLC columns (8 – 80 ml bed volume), B) FPLC Luer Lock Male connector, C) FPLC Luer Lock Female connector

Purification of

Agarose

GST-tagged proteins Glutathione Agarose
HIS-tagged proteins Ni-NTA Agarose
Ni-IDA Agarose
Co-IDA Agarose
Antibodies Protein A Agarose
mAB-Protein A Agarose (alkali-stable)
Protein G Agarose
Protein A/G Agarose

Where can I find more information about Jena Bioscience?

Visit the manufacturer page at www.stratech.co.uk/jena_bioscience, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


 

 

Biomatrica – The Biostability Company

The Problem

Presently, biological materials such as nucleic acids, proteins, cells, viruses and vaccines, are maintained and transported in cold environments (4ºC, -20ºC, -80ºC and liquid nitrogen) to preserve sample integrity. Multiple freeze-thaw cycles or prolonged exposure to increased temperatures degrade precious and labile biological materials. Sustaining cold environments requires constant energy supplies and resources that are not always readily available or reliable. Consequently, transporting and storing biological samples often times involves enormous expense and complicated logistics. Despite elaborate precautions taken to maintain sample integrity, it has been difficult to control all environmental variables – until now.

The Solution

Biomatrica® offers an innovative alternative to reliance on cold chain logistics. We have developed SampleMatrix® a proprietary core technology to stabilize and protect biological materials at room temperature without degradation. The SampleMatrix technology was designed by combining extremophile biology that enables long-term survival of organisms in extremely dry environments, with synthetic chemistry. By exploiting these unique characteristics, Biomatrica products enable researchers in academia, pharmaceutical drug development, and molecular diagnostics to stabilize, ship and store biological samples for long periods of time with complete and rapid sample recovery, and at very affordable costs.

Consistent sample maintenance allows for experimental reproducibility, and therefore ensures reliability, speeds time to results, greatly reduces costs, and is an environmentally sustainable alternative to all forms of cold storage. This technology can be applied to thousands of currently available products in the biotechnology, diagnostics and pharmaceutical industries, and has direct applications for billions of samples generated from genome mapping, and sample archiving used for personalized medicine and forensics analysis in law enforcement and the military.

Corporate Fact Sheet

For more information about Biomatrica products and services, please download our Corporate Fact Sheet.


Where can I find more information about Biomatrica?

Visit the manufacturer page at www.stratech.co.uk/biomatrica, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


 

 

Endothelial Cell Division Under Flow in SynVivo’s Microvascular Network

 

Time lapse video (~24 hours) of primary endothelial cells undergoing cell division and morphological changes under physiological flow conditions in the SynVivo microfluidic, cell-based assay platform.


Where can I find more information about SynVivo?

Visit the manufacturer page at www.stratech.co.uk/synvivo, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.