Product Focus: Polymer HRP and AP Detection Kits for Human

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Klear Human HRP Polymer and AP Polymer Kits are designed for the detection of Human primary antibody on Human or Primate tissue

Features

  • Specially designed to detect Human IgG antibody on Human tissue with clean background.
  • This polymer detection system is adsorbed to human serum protein to obtain superior  specificity and sensitivity.
  • As always GBI polymer kits are biotin free saving additional blocking step.

Simple Quick Protocol

  1. Primer and human primary antibody are incubated  overnight at 4℃.
  2. Human 1’Ab mixture is incubated on tissue (30 minutes to 2 hours)
  3. 10 minutes incubation for anti-Human polymer-HRP conjugate.
  4. 5 to 20 minute chromogen incubation. Select chromogen Kits: DAB, DAB-Ni, AEC, AP-Red+, Fast-Red, BCIP/NBT, AP-Blue.
Cat No. Name Chromogen Size(s)
D103-18 Klear Human HRP-Polymer DAB Kit DAB 18ml
D103-6 Klear Human HRP-Polymer DAB Kit DAB 6ml
D104-18 Klear Human HRP-polymer with AEC Kit AEC 18ml
D104-6 Klear Human HRP-polymer with AEC Kit AEC 6ml
D105-18 Klear Human AP-polymer with Fast Red Kit AEC 18ml
D105-6 Klear Human AP-polymer with AEC Kit AEC 6ml
D106-18 Klear Human AP-polymer with AP-Red+ Kit AP-Red+ 18ml
D106-6 Klear Human AP-polymer with AP-Red+ Kit AP-Red+ 6ml

Where can I find more information about SBI?

Visit the manufacturer page at www.stratech.co.uk/gbi-labs, email info@stratech.co.uk or call    +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.

New Supplier: Golden Bridge International, Inc. High Quality Immunohistochemistry (IHC) Products

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Stratech Scientific are please to introduce GBI Labs.  GBI Labs was founded, in 1993, for the purpose of supplying well-developed, affordable and unique biomedical reagents to research and diagnostic communities, biotech and pharmaceutical industries in the world. GBI Labs develops and manufactures high quality Immunohistochemistry (IHC) products used for human anatomy pathology, veterinary pathology, antibody screening for drug discovery, and cancer research.
GBI Labs offers many unique products including double staining kit for co-expression, triple staining kits, human antibody on human tissue screening kits, etc.

Key Product Offerings:

Polymer HRP and AP (non-biotin) multi color staining kits

Multi color staining allows reviewing 2-3 targeted proteins on one tissue section. GBI Labs’ Polink-DS (double staining) kits detect almost any two combinations of primary antibodies. Some kits are specially designed for reviewing co-expression on the same location in the tissue section.
GBI Labs’ Polink-TS (triple staining) kits allow detecting 3 antigens with 5-6 hours. Two combinations of colors (brown/green/red or brown/red/black) can be selected at customer’s choices.

Polymer HRP and AP (non-biotin) Detection Kits

GBI Labs provides high quality polymer (non-biotin) detection kits to detect mouse, rabbit, goat, rat, chicken, sheep, Armenian hamster, and guinea pig primary antibodies. All secondary antibodies used in our detection system are highly purified. Polink-1 (1-step polymer) kits give customer quick turnaround time. Polink-2 (2-step polymer) and Polink-2 Plus (2-step polymer) detection kits provide the best signal to noise ratio.

High Specificity Detection Kits for Rodent Tissue

GBI Labs’ ultra sensitive polymer HRP and AP kits use newest polymer conjugated secondary antibody and proprietary blocking buffer to detect mouse antibody on mouse tissue, rat antibody on rat tissue, rat antibody on mouse tissue, or mouse antibody on rat tissue with very clean background.

Where can I find more information about SBI?

Visit the manufacturer page at www.stratech.co.uk/gbi-labs, email info@stratech.co.uk or call   +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.

Product Focus: PrecisionX™ Cas9 SmartNuclease System Mammalian Genome Engineering with Guide RNAs

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Cas9 SmartNuclease™ System

  • Specific genome cleavage using gRNAs
  • Easy gene knockouts
  • Efficient genome editing
  • All-in-one Cas9-gRNA vectors

Genome Editing using guide RNAs The recent discovery of the type II prokaryotic CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system, originally discovered in the bacterium Streptococcus pyogenes that works as a mechanism to defend against viruses and foreign DNA, has provided a revolutionary tool for targeted genome engineering with simple elegance.

The system uses a nuclease, CRISPR-associated (Cas9), that complexes with small RNAs as guides (gRNAs) to cleave DNA in a sequence-specific manner upstream of the protospacer adaptor motif (PAM) in any genomic location. The CRISPR-Cas9 system is comprised of separate guide RNAs known as the crRNA and tracrRNA. These two separate RNAs have been combined into a single RNA to enable site-specific mammalian genome cutting through the design of a short guide RNA. SBI has built a series of all-in-one CRISPR-Cas9 cloning vectors with different promoter options. These cloning vectors will express a human codon-optimized Cas9 nuclease as well as any gRNA sequence of your choice linked to the tracrRNA. Simply design the gRNA for the genome target DNA sequence and clone into the Cas9 SmartNuclease™ vector for efficient genome engineering. The PrecisionX™ Cas9 SmartNuclease™ Vector products are the subject of pending US and foreign patent applications.

Online Resources

Technical references

Carr PA, Church GM. Genome engineering. Nat Biotechnol. 2009 Dec;27(12):1151-62.

Bhaya D, Davison M, Barrangou R. CRISPR-Cas systems in bacteria and archaea: versatile small RNAs for adaptive defense and regulation. Annu Rev Genet. 2011;45:273-97.

Terns MP, Terns RM. CRISPR-based adaptive immune systems. Curr Opin Microbiol. 2011 Jun;14(3):321-7.

Makarova KS, Haft DH, Barrangou R, Brouns SJ, Charpentier E, Horvath P, Moineau S, Mojica FJ, Wolf YI, Yakunin AF, van der Oost J, Koonin E. Evolution and classification of the CRISPR-Cas systems. Nat Rev Microbiol. 2011 Jun;9(6):467-77.

Wiedenheft B, Sternberg SH, Doudna JA. RNA-guided genetic silencing systems in bacteria and archaea. Nature. 2012 Feb 15;482(7385):331-8.

Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science. 2012 Aug 17;337(6096):816-21.

Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM. RNA-guided human genome engineering via Cas9. Science. 2013 Feb 15;339(6121):823-6.

Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Multiplex genome engineering using CRISPR/Cas systems. Science. 2013 Feb 15;339(6121):819-23.

Jinek M, East A, Cheng A, Lin S, Ma E, Doudna J. RNA-programmed genome editing in human cells. Elife. 2013;2:e00471. doi: 10.7554/eLife.00471.

Where can I find more information about SBI?

Visit the manufacturer page at www.stratech.co.uk/SBI, email info@stratech.co.uk or call  +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.

Product Focus: Endotoxin Free Growth Factors from Abnova

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Endotoxins, also known as lipopolysaccharides (LPS) are produced and released by Gram-negative bacteria, such as E. coli. Currently, acceptable level of endotoxin traces in experiments is 0.1 ng endotoxin per ug growth factor (~1 EU/ug). However, results from hESC research experiments have shown that it can have profound effects on stem cell behavior in culture even at this level of endotoxin.

In an Embryoid Bodies (EBs) based in vitro stem cell study recently published in Regenerative Medicine*, endotoxin exposure, as low as 0.005 ng/ml, was demonstrated to impede stem cell differentiation by inhibiting mesoderm formation. Sivasubramaniyan K, et. al. found that the LPS-treated EBs were selectively silenced for mesoderm markers and failed to differentiate into functional osteoblasts. This concentration correlates to adding growth factors with 0.1 ng/ug (~1 EU/ug) bacterial endotoxins to the stem cell culture. (Up to 5 commercial growth factors were used for direct cell differentiation in concentration of 10 ng/ml each, and each growth factor contains close to 0.1 ng/ug endotoxin. Total LPS in the cell culture is close to 0.005 ng/ml. )

*Sivasubramaniyan K, et. al. Regen Med. 2008, 3(1): 23-31

Data1
Abnova provides endotoxin-free, animal-free, and serum-free growth factors expressed in barley seed, bypassing the use of bacterial or animal cell systems. Using these growth factors from Abnova will greatly reduce your risks in comparison with other commercial growth factors that are produced by gram-negative bacteria, such as E. coli.
Data2
Abnova Endotoxin Free Growth Factors include:
Human Growth Factors Mouse Growth Factors
BMP2 CSF1 CSF2 CSF3 FGF2 FGF7 Lif
FLT3LG GDNF IFNG IL2 IL3 IL4 Vegfa
INHBA INHBB LIF TNF VEGFA

Where can I find more information about Abnova?

Visit the manufacturer page at www.stratech.co.uk/abnova, email info@stratech.co.uk or call  +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.

Product News: More on the Neurobiology of Itch from Neuromics

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Here chronic itch was studied in Macque Monkeys over a period of 4 years. The expression patterns of GRP, GRPR and PGP9.5 were accessed by immunohistochemistry: Leigh A Nattkemper, Zhong-Qiu Zhao, Anna J Nichols, Alexandru D P Papoiu, Carol A Shively, Zhou-Feng Chen and Gil Yosipovitch. Over-Expression of the Gastrin-Releasing Peptide in Cutaneous Nerve Fibers and its Receptor in Spinal Cord in Primates with Chronic Itch. Journal of Investigative Dermatology accepted article preview 4 April 2013; doi: 10.1038/jid.2013.166.

Images: Double labeling of PGP9.5 and GRP in skin of primates representing mild, moderate and severe itch. Primates with higher scratching severity showed in increase in the co-localization of PGP9.5 and CRP at the dermal-epidermal junctions (arrows).

In addition to increased PGP9.5/GRP expression in the skin, similar results were shown for expression of GRP/GRPR in DRGs. This makes GRP and its receptor candidate drug targets for chronic itch or pruritis in humans.

Where can I find more information about Neuromics?

Visit the manufacturer page at www.stratech.co.uk/neuromics, email info@stratech.co.uk or call    +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe.

Please contact us to find out which product ranges we can supply in your country.

Product Focus: STELLUX™ Chemi Rodent Insulin ELISA

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Introducing ALPCO’s newest addition to their Diabetes & Obesity portfolio, our STELLUX Rodent Insulin Chemiluminescent ELISA.

Chemiluminescent assays provide rapid and sensitive (low signal-to-noise) detection, exhibit a wide dynamic range and are comparable to electrochemiluminescent assays in terms of performance.

Confidently detect from 0.1 to 150 ng/mL in 5 mL of serum or plasma for both mouse and rat:

  • Broad dynamic range
  • Small sample size
  • Elimination of dilutions
  • Short 2 hour incubation

The ALPCO STELLUX™ Rodent Insulin ELISA kit quantifies the concentration of the insulin protein products of the mouse I, mouse II, rat I and rat II proinsulin genes in the range of 0.1 – 150 ng/mL from 5µL of serum or plasma sample. Utilizing a dual-monoclonal antibody sandwich format, tri-level control set, and 96-well microplate comprising removable strips, a single STELLUX™ Rodent Insulin ELISA kit has the performance characteristics and flexibility necessary to confidently measure up to 36 samples in duplicate. The shelf-life of the components and the resealable microplate pouch allow for convenient storage for future use if the entire kit is not needed at one time.

Catalog Number: 80-INSMR-CH01
Description: STELLUX™ Rodent Insulin ELISA
Species Types: Mouse, Rat
Sample Types: Plasma, Serum
Sample Sizes: 5 µL
Available Sizes: 96 Wells
Range: 0.1 – 150 ng/mL (Requires a chemiluminescent reader)
Sensitivity: 0.096 ng/mL
Incubation: 2 hour(s)
Protocol: STELLUX™ Rodent Insulin ELISA

Where can I find more information about ALPCO Diagnostics?

Visit the manufacturer page at www.stratech.co.uk/alpco, email info@stratech.co.uk or call   +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.

Product Focus: Human Osteopontin ELISA

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The Osteopontin ELISA from ALPCO Diagnostics is for the quantification of osteopontin in human plasma and urine.

Osteopontin is a member of the SIBLING (Small Integrin-Binding Ligand N-linked Glycoprotein) family. It is 300 amino acids in length, is expressed in a variety of tissues and can be post-translationally modified. The full-length protein is detectable in plasma, urine and breast milk. Serum osteopontin is prone to cleavage during clotting and therefore is not a preferred sample type. Osteopontin plays a role in a number of physiological functions including bone remodeling, immune function, apoptosis and cell signaling. Studied across a range of therapeutic areas, there is interest in identifying clinical applications for osteopontin as it relates to cancer, autoimmune disease, bone disease, renal disease, allergy and asthma, among others.

Catalog Number: 41-OPNHU-E01
Description: Osteopontin ELISA
Species Types: Human
Sample Types: Heparin Plasma, Urine
Sample Sizes: 10 µL
Available Sizes: 96 Wells
Range: 0.31 – 20 ng/mL
Sensitivity: 0.05 ng/mL
Incubation: 3 hour(s)
Protocol: Osteopontin ELISA

Where can I find more information about ALPCO Diagnostics?

Visit the manufacturer page at www.stratech.co.uk/alpco, email info@stratech.co.uk or call  +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.

 

Product Focus: Cal-520™, the Best Green Fluorescent Calcium Dyes

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Cal-520™ provides the most robust homogeneous fluorescence-based assay tool for detecting intracellular calcium mobilization. Cal-520™ AM is a new fluorogenic calcium-sensitive dye with a significantly improved signal to background ratio and intracellular retention compared to the existing green calcium indicators (such as Fluo-3 AM and Fluo-4 AM). The higher signal/background ratio and better intracellular retention make the Cal-520™ calcium assay a robust tool for evaluating GPCR and calcium channel targets as well as for screening their agonists and antagonists.

AAT Bioquest’s preliminary in-house research indicated that Cal-520™ AM can be readily loaded to a guinea pig heart and stays there for a few hours in the absence of probenecid. The calcium signal can be readily monitored using Cal-520™ AM while it is difficult to observe the calcium signal under the same conditions using other green calcium dyes such as Fluo-3 AM and Fluo-4 AM.

Key Features

  • Better Intracellular Retention, Cal-520™ AM is well retained in live cells.
  • Higher Sensitivity, Cal-520™ AM has the highest signal/background ratio.
  • Convenient, Cal-520™ AM has almost identical spectra to those of Fluo-4 AM.

Products

  • Cat. No. 21131 Cal-520™ AM
  • Cat. No. 21141 Cal-520™, potassium salt
  • Cat. No. 21136 Cal-520™, sodium salt
  • Cat. No. 21142 Cal-520FF™ AM
  • Cat. No. 21144 Cal-520FF™, potassium salt
  • Cat. No. 20501 Cal Green™-1 (equivalent to Calcium Green™-1 AM)
  • Cat. No. 20500 Cal Green™-1, hexapotassium salt (equivalent to Calcium Green™-1)

Where can I find more information about AAT Bioquest?

Visit the manufacturer page at www.stratech.co.uk/aat_bioquest, email info@stratech.co.uk or call  +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.

Product Focus: IPS Specialized Growth and Cell Passaging Tissue Culture Media from Cellular Engineering Technologies

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IPS cells are fastidious cells that require regular maintenance and should only be grown in CET’s proprietary IPS cell growth media. CET’s IPS growth media has been uniquely formulated to restrict cell differentiation, promote robust growth and prevent cell death. CET’s growth media has been uniquely formulated so that media only has to be changed every other day, instead of the conventional everyday requirement. This decreases the supply and labor cost of growing IPS cells.

CET has created an enzymatic dissociation media in which cultured IPS cells are incubated at 37 degrees celsius for 1 hour and the underlying feeders are easily separated from the IPS colonies, which prevents feeder cell contamination. Further, the colonies are maintained in an aggregate state, which facilitates cell colony passaging on new feeders and avoids spillage of non-pluripotent IPS cells.

Catalogue No.
Description
Quantity
CET.IPS.Media-200
IPS Base Media
200 mL
CET.IPS.Supp-50
IPS Supplement
50 mL
CET.IPS.PASS-100
IPS Cell Passaging Media
100 mL

For further information on growing and passaging CET’s IPS cell lines, please download and follow the instructions described in the manual entitled, Cellular Engineering Technologies Inc. Guide to IPS Cell Culture.

Where can I find more information about CET?

Visit the manufacturer page at www.stratech.co.uk/cellular_engineering_technology, email info@stratech.co.uk or call  +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.

Product Focus: Contamination Control Kits – Nuclease and Protease Control from Jena Biscience

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Monitoring nuclease and protease in diagnostics and biotechnological production is an important part of in-process control. 

Jena Biscience currently provides the following products for this purpose.

DNase+RNase Detection Kit – Fluorescence based detection of DNase and RNase activities
Product Cat. No. Amount
S pack PP-407S 20 reactions
L pack PP-407L 100 reactions
DNase+RNase Detection Kit provides optimized reagents for highly sensitive, fast and easy-to-perform DNase and RNase single step detection assay. The kit allows the detection of lowest amounts of RNase A, RNase T1, RNase 1 and other RNA degrading enzymes as well as DNase I and other ss- or ds-DNA degrading enzymes. It is ideal for contamination testing of a few samples as well as for routine process monitoring.
Using the DNase+RNase Detection Kit as little as 0.2 pg RNae A and
2 munits (approx. 4 pg) of DNase I can be detected within 20-30 min.
DNase Detection Kit
Fluorescence based detection of DNase activity
Product Cat. No. Amount
S pack PP-402S 20 reactions
L pack PP-402L 100 reactions
DNase Detection Kit provides optimized reagents for a highly sensitive, fast and easy-to-perform DNase detection assay. The kit allows the detection of lowest amounts of DNase I and other ss- or ds-DNA degrading enzymes. It is the ideal tool for contamination testing of a few samples or for routine process monitoring.
Using the DNase Detection Kit as little as 2 munits (approx. 4 pg) of DNase I can be detected within 20-30 min.

P-CHECK® Protease Detection Kit
Product Cat. No. Amount
P-CHECK® is a trademark of PANATecs, Germany
P-CHECK® Protease Detection Kit is based on the FRET technology (Fluorescence Resonance EnergyTransfer). This test procedure guarantees maximal sensitivity for proteases down to the picomolar range.

Protease Detection Kit
Product Cat. No. Amount
S pack PP-404S 50 reactions
L pack PP-404L 200 reactions
Protease Detection Kit detects a wide variety of proteases, including serine proteases, cysteine proteases and acid proteases in biological samples. The kit is based on a labeled casein derivative as protease substrate, to be incubated with the sample for 2 to 20 hours at 37°C. Active proteases cleave the labeled casein into peptides which are measured after centrifugation in the supernatant at 492 nm.

Where can I find more information about Jena Bioscience?

Visit the manufacturer page at www.stratech.co.uk/jena_bioscience, email info@stratech.co.uk or call   +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.