Supplier Focus: SoluLink – Magnetic Beads & Agarose

Highest biotin-binding streptavidin magnetic beads and agarose on the market

Over the past 10 years, Solulink’s streptavidin-coupled NanoLink™ and MagnaLink™ Beads have been widely used in types of organizations and are regularly cited in papers for diverse manual and automated applications. Watch the video to learn more:

Solulink offers streptavidin magnetic beads and agarose with the highest biotin-binding capacity on the market – beads with as much as 12X higher binding capacity and agarose with 20% lower price. Higher binding translates into reduced bead mass or agarose required to immobilize a biotinylated sample and lower background noise from nonspecific binding, resulting in better signals and lower net costs.

Molecule

NanoLink (0.8 um) Binding Capacity

MagnaLink (2.8 um) Binding Capacity

Free biotin
>14 nmol/mg
>12 nmol/mg
Biotinylated oligo (23-mer)
>2.5 nmol/mg
>0.8 nmol/mg
Biotinylated IgG (3 biotins per IgG)
>250 ug/mg
>112.6 ug/mg

The secret is in the crosslinking
The key to high biotin binding is in the unique covalently crosslinked streptavidin, based on Solulink’s proprietary linking technology. The high surface area, when combined with our efficient linking chemistry, produces a consistent, ultra-high, biotin-binding bead.

Applications

  • Immobilization of biotinylated biomolecules or drugs
  • Immunoprecipitation, ChIP, cell capture, and others
  • Cell-surface labeling with biotinylation reagents, followed by precipitation with streptavidin
  • Recovery of biotinylated DNA for dideoxy sequencing


Streptavidin Agarose Ultra Performance

High biotin-binding streptavidin agarose

Solulink’s Streptavidin Agarose Ultra Performance™ provides high biotin binding at a low price. We used Solulink’s proprietary linking technology coupled with a 6% highly crosslinked agarose to boost the biotin binding capacity of the high specific activity streptavidin. This ideal combination provides a biotin binding capacity of >330 nmol/mL of resin—one of the highest loading-capacity products currently available. Use Solulink’s Streptavidin Agarose Ultra Performance™ for improved recovery of any biotinylated biomolecule and you can lower nonspecific binding, lower costs, and get better results.

Application Note: How to Capture at Least 2x More Antigen in an Immunoprecipitation

Comparative Binding Data

Advantages

  • High binding capacity – Higher biotin binding capacity at >20% lower price
  • Crosslinked agarose – Solulink’s proprietary linker enables higher binding capacity, lower background, and less leaching
  • Multiple sizes – Available in 2 mL, 5 mL, 10 mL, and bulk quantities

Applications

  • Immobilization of biotinylated biomolecules or drugs
  • Immunoprecipitation, ChIP, cell capture, and others
  • Cell-surface labeling with biotinylation reagents, followed by precipitation with streptavidin
  • Recovery of biotinylated DNA for dideoxy sequencing

Products

Cat. No. Product Name Size
M-1002-010-SLK NanoLink™ Streptavidin Magnetic Beads 0.8 μm 1 mL at 10 mg/mL
M-1002-020-SLK NanoLink™ Streptavidin Magnetic Beads 0.8 μm 2 mL at 10 mg/mL
M-1002-050-SLK NanoLink™ Streptavidin Magnetic Beads 0.8 μm 5 mL at 10 mg/mL
M-1002-100-SLK NanoLink™ Streptavidin Magnetic Beads 0.8 μm 10 mL at 10 mg/mL
M-1002-1000-SLK NanoLink™ Streptavidin Magnetic Beads 0.8 μm 100 mL at 10 mg/mL: Please Inquire for Pricing
M-1001-010-SLK NanoLink™ 4FB Magnetic Beads 0.8 μm 1 mL at 10 mg/mL
M-1000-001-SLK NanoLink™ Amino Magnetic Beads 0.8 μm 1mL at 10 mg/mL
M-1003-010-SLK MagnaLink™ Streptavidin Magnetic Beads 2.8 µm 1 mL at 10 mg/mL
M-1003-020-SLK MagnaLink™ Streptavidin Magnetic Beads 2.8 µm 2 mL at 10 mg/mL
M-1003-050-SLK MagnaLink™ Streptavidin Magnetic Beads 2.8 µm 5 mL at 10 mg/mL
M-1003-100-SLK MagnaLink™ Streptavidin Magnetic Beads 2.8 µm 10 mL at 10 mg/mL
M-1003-1000-SLK MagnaLink™ Streptavidin Magnetic Beads 2.8 µm 100 mL at 10 mg/mL
M-1004-010-SLK MagnaLink™ 4FB Magnetic Beads 2.8 μm 1 mL at 10 mg/mL
M-1005-010-SLK MagnaLink™ Amino Magnetic Beads 2.8 µm 1mL at 10 mg/mL
N-1000-002-SLK Streptavidin Agarose Ultra Performance 2 mL
N-1000-005-SLK Streptavidin Agarose Ultra Performance 5 mL
N-1000-010-SLK Streptavidin Agarose Ultra Performance 10 mL

Select Citations from Top Researchers

NanoLink™ and MagnaLink™ Streptavidin Magnetic Beads

    • Das A, Goldstein JL, Anderson DD, et al. Use of mutant 125I-perfringolysin O to probe transport and organization of cholesterol in membranes of animal cells. Proc. Natl. Acad. Sci. U.S.A. 2013 Jun 25;110(26):10580–10585. Link
    • Steele S, Brunton J, Ziehr B, et al. Francisella tularensis Harvests Nutrients Derived via ATG5-Independent Autophagy to Support Intracellular Growth. PLoS Pathog. 2013 Aug;9(8):e1003562. Link
    • Flanigon J, Kamali-Moghaddam M, Burbulis I, et al. Multiplex protein detection with DNA readout via mass spectrometry. N Biotechnol. 2013 Jan 25;30(2):153–158. Link
    • Tanabe C, Maeda T, Zou K, et al. The Ubiquitin Ligase Synoviolin Up-regulates Amyloid β Production by Targeting a Negative Regulator of γ-Secretase, Rer1, for Degradation. J. Biol. Chem. 2012 Dec 28;287(53):44203–44211. Link
    • Fowler DM, Araya CL, Fleishman SJ, Kellogg EH, Stephany JJ, Baker D, Fields S. High-resolution mapping of protein sequence-function relationships. Nature Methods 2010;7(9):741-6. Link
    • Kuzmin A, Poloukhtine A, Wolfert MA, Popik VV. Surface functionalization using catalyst-free azide-alkyne cycloaddition. Bioconjug Chem. 2010;21(11):2076-85. Link
    • Fan WQ, Morinaga H, Kim JJ, Bae E, Spann NJ, Heinz S, Glass CK, Olefsky JM. FoxO1 regulates Tlr4 inflammatory pathway signalling in macrophages. The EMBO Journal 2010;29(24):4223-36. Link
    • Branchini BR, Ablamsky DM, Rosenberg JC. Chemically modified firefly luciferase is an efficient source of near-infrared light. Bioconjug Chem. 2010;21(11):2023-30. Link
    • Jensen RB, Carreira A, Kowalczykowski SC. Purified human BRCA2 stimulates RAD51-mediated recombination. Nature 2010;467(7316):678-683. Link
    • Fowler DM, Araya CL, Fleishman SJ, Kellogg EH, Stephany JJ, Baker D, Fields S. High-resolution mapping of protein sequence-function relationships. Nature Methods 2010;7(9):741-6. Link
    • Kuzmin A, Poloukhtine A, Wolfert MA, Popik VV. Surface functionalization using catalyst-free azide-alkyne cycloaddition. Bioconjug Chem. 2010;21(11):2076-85. Link
    • Fan WQ, Morinaga H, Kim JJ, Bae E, Spann NJ, Heinz S, Glass CK, Olefsky JM. FoxO1 regulates Tlr4 inflammatory pathway signalling in macrophages. The EMBO Journal 2010;29(24):4223-36. Link
    • Branchini BR, Ablamsky DM, Rosenberg JC. Chemically modified firefly luciferase is an efficient source of near-infrared light. Bioconjug Chem. 2010;21(11):2023-30. Link
    • Jensen RB, Carreira A, Kowalczykowski SC. Purified human BRCA2 stimulates RAD51-mediated recombination. Nature 2010;467(7316):678-683. Link

Where can I find more information about Solulink?

Visit the manufacturer page at www.stratech.co.uk/solulink, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


Product Focus: Cell Co-Culture Idealised in a Microfluidic Platform

Intended to mimic the formation of and transport across tight and gap junctions such as the blood-brain barrier and other endothelial/tissue interfaces, the idealised co-culture constructs are available with several options for channel size, tissue chamber size and scaffolding, and barrier design.

Use idealised co-culture assays to mimic the cellular make up in vivo. Investigate cell-cell interactions and perfusion vs. diffusion based affects. Analyze the experiments in real-time for all the cell populations.

Click on images for larger view


Slit Barrier Option

This device utilizes slits and gaps to form the barrier region between the outer channel and inner chamber.Standard design parameters available are:

  • Outer Channel Width (OC): 100 μm or 200 μm
  • Travel Width (T): 50 μm or 100 μm
  • Slit Spacing (SS): 50 μm or 100 μm
  • Slit Width (WS): Variable

IMN2/IMN3 (Slits)

IMN2/IMN3 Chamber Zoom

IMN2/IMN3 Slit Details


Where can I find more information about SynVivo?

Visit the manufacturer page at www.stratech.co.uk/synvivo, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


News: Rapid Fecal Calprotectin (FC) Analysis: Point of Care Testing for Diagnosing Early Necrotizing Enterocolitis

An exciting recent publication from Dr. Alona Bin-Nun and Dr. Cathy Hammerman on the application of the BÜHLMANN Fecal Calprotectin ELISA and Quantum Blue® Calprotectin Rapid Test for identifying Necrotizing Enterocolitis (NEC). This publication demonstrates the comparability and utility of the Rapid Test to provide a quick turnaround time of Fecal Calprotectin value in the premature neonates at high risk for NEC.


Citation

Bin-Nun A, Booms C, Sabag N, Mevorach R, Algur N, Hammerman C. Rapid Fecal Calprotectin (FC) Analysis: Point of Care Testing for Diagnosing Early Necrotizing Enterocolitis. Am J Perinatol. 2014 Aug 11. [Epub ahead of print] PMID: 25111039


Where can I find more information about ALPCO Diagnostics?

Visit the manufacturer page at www.stratech.co.uk/alpco, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


Accelerate Drug Discovery with In-Depth Compound Selectivity Profiling

Uncovering the target selectivity profile of any small molecule is of fundamental clinical importance in the drug development process. These studies provide useful information about novel compounds, including putative mechanism of action and potential off-target effects, leading to the selection of the most viable clinical candidates.

Spanning the spectrum of cell signaling mechanisms, SignalChem offers the most diverse target offering; both wild-type kinases and the most ‘complete’ range of mutant kinases are available, allowing physiologically relevant results. Our in-house expertise and high throughput methodologies ensure dependable and reproducible data with rapid turnaround times.

Benefits

  • Greater Confidence in Your Data
  • Reliable Results for Lead Discovery
  • Efficient and Effective Drug Development

Features

  • High Signal-to-Noise Ratio
  • Radiometric Enzyme Assay
  • Two-Week Turnaround Time

Table1: Enzymes Available for Compound Profiling

Figure 1. Sample Inhibitory Compound Profile Highlighting Dose-Dependent Effects

Figure 2. Radiometric Assay of Protein Kinases: The Gold Standard for Measuring Kinase Activity


Where can I find more information about SignalChem?

Visit the manufacturer page at www.stratech.co.uk/signalchem, email info@stratech.co.uk or call +44 (0) 1638 782600

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


Product Focus: Diaplate – Disposable Micro-Dialysis Plate

Stratech Scientifc provides through Jena Bioscience SWISSCI’s disposable Diaplate. This 96 well micro-dialysis plate is ideal for the desalting of protein from very small volumes of up to a maximum of 3.2 µl. The sample solution is pipetted into each of the 96 wells with up to 0.35 ml dialysis solution on the opposite side used for exchange. The Diaplate is ANSI/SLAS 1-2004 standards providing high throughput use with robotic dispensing. Dialysis is recommended for 2-3 hours and then the dialysis buffer is changed being left for another 2-3 hours to achieve over 90% exchange. Further dialysis steps can be carried out as required. Incubation, rocking table for speed and moisture chamber for full humidification can be applied to the experiment if desired.
Each of the 96 wells has a separate regenerated cellulose membrane inside meaning no cross contamination or leakage between wells. The Swissci Diaplate comes as standard with a MWCO of 10 kDa, others are available upon request.


The Diaplate Kit includes

  • Diaplate with 200 Micron Pressure Adhesive Spacer
  • 200 Micron UVP Cover Film
  • Engraved UV Protective Cover
  • Sealing Paddle

Applications

  • Protein and peptide purification/concentration
  • Variation of crystallisation conditions for crystal growth
  • Removal of dyes after protein labelling
  • Electroelution of proteins
  • Protein and DNA desalting/buffer exchange
  • Protein in vitro translation
  • Glycoprotein modification and engineering
  • Enzyme activity/binding assays/detergent removal

Products

Product Cat. No. Amount Downloads
Diaplate Kit
96 well
MWCO 10 kDa
X-W82010 3 pcs.

Where can I find more information about Jena Bioscience?

Visit the manufacturer page at www.stratech.co.uk/jena_bioscience, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


Product Focus: Screening – Thermofluor Screens

The Thermofluor Screens JBS FUNDAMENT and JBS SPECIFIC allow identification of protein-stabilizing buffer conditions which is pivotal for protein purification, characterization and crystallization.
Undesired overlay of screening of interdependent variables is prevented by strictly categorizing stability screening of proteins into

  1. FUNDAMENTAL factors that influence the whole protein molecule (pH and ionic strength)
  2. SPECIFIC factors that affect energetically important hot spots on the protein (substrates and their analogs, cations, anions, …)
 

The protein’s melting temperature (Tm) is used as reporter for protein stability and determined by a thermal shift assay (96-well plate format) monitoring the unfolding of a protein in a temperature-dependent manner. The higher the Tm, the higher is the thermostability of the protein in that specific environment.


Products

Product Cat. No. Amount Downloads
JBS FUNDAMENT Thermofluor Screen CS-330-JEN 1 Kit
JBS SPECIFIC Thermofluor Screen CS-331-JEN 1 Kit

References

  • Ericsson et al. (2006) Thermofluor-based high-throughput stability optimization of proteins for structural studies. Anal. Biochem.357(2):289.
  • Reinhard et al. (2013) Optimization of protein buffer cocktails using Thermofluor. Acta Cryst. F69:209.
  • Niesen et al. (2007) The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability. Nat. Protoc.2(9):2212.

Where can I find more information about Jena Bioscience?

Visit the manufacturer page at www.stratech.co.uk/jena_bioscience, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


Supplier Focus: Dilyx Biotechnologies – Protein Solubility Screening Kits

OptiSol Protein Solubility Kits help researchers identify formulations that protect target protein from aggregation, or to solubilize an aggregated protein sample.

  • Quickly evaluate aggregation behavior as a function of pH, salt and additives
  • Assay more than 90 different conditions in parallel and label-free
  • Simple mix and spin protocol, no ultracentrifugation required
  • Opportunity to rescue reversibly aggregated protein samples
  • Protect your protein from aggregation under stress*
  • Quickly find trends with the Protein Dashboard

Proteins aggregate. This undesired process is often caused by elevated temperature, vigorous stirring, addition of ligands or molecular binding partners or just by mere storage of a protein sample over a period of time. Protein aggregation can often be avoided with proper choice of pH, salt or stabilizing additives. This OptiSol Protein Solubilization Screening Kit contains a systematically varied array of buffers (from pH 3 to pH 10) and a series of solubility enhancers (salts, amino acids, sugars, polyols, reducing reagents) that enable the determination of conditions under which a particular protein sample is protected from aggregation or can be de-aggregated. A total of 90 different formulations with solubility enhancers can be tested in one single, label-free experiment; the remaining 6 experiments are positive and negative control experiments.

Soluble proteins pass filters, aggregated proteins don’t. This simple feature is exploited to in the filtration step. The wells that contain soluble proteins will yield protein after the filtration process; those with protein aggregates will not contain any protein.

After running the Protein Solubilization Kit you have established conditions that keep your protein target in solution and you know conditions at which your protein aggregates. Then choose conditions to obtain a “well behaved protein”.

  • Better and faster than a conventional trial-and-error testing (less than 1 h)
  • Low protein consumption consumes less than 10 uL for each protein solubility data point. The protein quantity needs to be matched with your target detection assay
  • Convenient: get a lot of information on protein solubilization in one go. Utilize the target specific assay of your own choice. The OptiSol Protein Solubility Screening kit is compatible with many functional assays, SDS-PAGE, Western blot, mass spectrometry and fluorescence-based protein detection.

OpitSol 30 or 200?

The OptiSol Kit comes with two different filters. Your choice between OptiSol 30 and OptiSol 200 will depend on the expected MW of the solubilized protein particle.


Applications for the Protein Solubility Screening Kit OptiSol

The OptiSol Protein Solubility Screening Kit can be used to protect target proteins against a variety of aggregation-inducing stresses. Proteins often aggregate when exposed to elevated temperature, when they are stored for longer periods of time, when protein solutions are repeatedly frozen and thawed or when the liquid is exposed to high shear forces or intense light. Oxidation by air, reactive reagents or surface exposure provide further sources of protein aggregation.

These stressors can be used to assess the susceptibility of a particular protein sample and identify protective agents.

If you challenge is

  • Undesired antibody or protein aggregation
  • Reversible precipitation of proteins during elution off a purification column
  • Insoluble protein at neutral pH

If you need to

  • Screen different buffers to maintain your protein stable at high concentrations
  • Keep an antigen soluble while removing certain components from the buffer
  • Separate bound antigen-antibody complex into unbound forms
  • Find optimal conditions for long term stability of proteins following purification.
  • Optimize purification conditions

the OptiSol Protein Solubility Screening Kit could take you a huge step forward.


Examples for experimental stresses that can be applied to protein solutions after they are mixed with the OptiSol formulations.


Where can I find more information about Dilyx Biotechnologies LLC?

Visit the manufacturer page at www.stratech.co.uk/dilyx_biotechnologies, email info@stratech.co.uk or call    +44 (0) 1638 782600

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


Supplier Focus: Jena Bioscience – Trap the Enzyme – with Non-Hydrolyzable Nucleotides

The enzymes involved in hydrolysis of nucleoside triphosphates are a major focus of biochemical research, targeting the understanding and treatment of cellular processes involved in diseases such as cancer, viral infections, cardiovascular and motional disorders. To elucidate the mechanisms of action of these enzymes, it is often desirable to trap the enzymes in their triphosphate-bound state.

Since natural nucleotides are hydrolyzed at rates usually preventing such studies, nucleotide analogs are used that are hydrolyzed at much smaller rates or that are even completely resistant to hydrolysis. The position of the modification within the triphosphate exerts influence on its binding affinity to a certain enzyme and its hydrolysis rate.

Jena Bioscience provides a number of nucleotides to assist with such studies.  Non-hydrolyzable nucleotides have become indispensable tools in research of nucleotide-utilizing enzymes such as polymerases, ligases, kinases, GTPases.

They are used as:

  • inhibitors and biochemical probes
  • in kinetic investigations
  • for crystallographic studies and
  • as potential therapeutics[1].

Reference
[1] Korhonen et al. (2014) Phosphate analogues in the dissection of mechanism. Current Opinion in Chemical Biology 21:63.


Products

Adenosine Nucleotides
Product Cat. No. Amount Downloads
ATPγS, 5 mg pack NU-406-5 5 mg
ATPγS, 25 mg pack NU-406-25 25 mg
ATPγS, 50 mg pack NU-406-50 50 mg
Mant-ATPγS, S pack NU-232S 20 Units
Mant-ATPγS, L pack NU-232L 5 x 20 Units
2-Iodo-ATPγS, 1 mg pack
(2I-ATPγS)
NU-905-1 1 mg
2-Iodo-ATPγS, 5 mg pack
(2I-ATPγS)
NU-905-5 5 mg
N6-Benzyl-ATPγS, pack
6-Bn-ATPγS
NU-241 3,4 mg
(approx. 5 µmol)
Guanosine Nucleotides
Product Cat. No. Amount Downloads
GTPγS, 2 mg pack NU-412-2 2 mg
GTPγS, 10 mg pack NU-412-10 10 mg
GTPγS, 20 mg pack NU-412-20 20 mg
Mant-GTPγS, S pack NU-209S 10 Units
Mant-GTPγS, L pack NU-209L 5 x 10 Units
Xanthosine Nucleotides
Product Cat. No. Amount Downloads
XTPγS, 1 mg pack NU-404-1 1 mg
XTPγS, 5 mg pack NU-404-5 5 mg
Inosine Nucleotides
Product Cat. No. Amount Downloads
ITPγS, S pack NU-413S 20 Units
ITPγS, L pack NU-413L 5 x 20 Units
Mant-ITPγS, S pack NU-215S 10 Units
Mant-ITPγS, L pack NU-215L 5 x 10 Units
Uridine Nucleotides
Product Cat. No. Amount Downloads
UTPγS, 1 mg pack NU-416-1 1 mg
UTPγS, 5 mg pack NU-416-5 5 mg

Where can I find more information about Jena Bioscience?

Visit the manufacturer page at www.stratech.co.uk/jena_bioscience, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


Product Focus: VeriPlex Human Cytokine 16-Plex ELISA Kit

VeriPlex Human Cytokine 16-Plex ELISA Kit is a chemiluminiscence based sandwich enzyme linked immunosorbent assay (ELISA) that can simultaneously quantitate Type I, II & III IFNs (IFN-α; IFN-β; IFN-γ; IFN-λ1/2/3) and 12 other key innate and adaptive immunity cytokines (TNF-α, IL-1α, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-15, IL-17, and IL-23) in low volumes of serum, plasma and cell culture supernatants. The kit employs a cost-effective multiplex ELISA technology in which 16 distinct capture antibodies are used in each well of a 96-well plate in a 4×4 array, and provides unique chemiluminescent results on each analyte within a sample in various matrices. The assay is compatible with multiple matrices including tissue culture media, human serum, human plasma and buffers.

This product has been developed to work in samples that are known to measure false positives in immunoassays such as samples containing human anti-mouse antibodies, anti-nuclease antibodies, and rheumatoid factor. The use of this product has been evaluated in serum from Rheumatoid Arthritis (RA) and Systemic Lupus Erythematosus (SLE) patients.

  • Sample volume required: 50 μl to quantify all 16 analytes
  • Compatible with serum and plasma from auto-immune disease patients
  • Detects 14 subtypes and alleles of IFN-α at different sensitivities
  • Detects all 3 subtypes of IFN-λ

Representative Standard Curves


Product Features

  • Cytokine Multiplex ELISA kit with unique and customizable array of molecules including Type I, II & III IFNs, ILs, TNF Alpha and more
  • Simultaneous quantification of up to 16 pro-inflammatory and anti-inflammatory cytokines in a single 50 μl sample
  • Provides analyte sensitivity as low as 5 pg/ml
  • Reproducible results with excellent intra- and inter-assay CVs (< 15%) for most analytes in difficult matrices, including autoimmune serum and plasma

Documentation

References


Where can I find more information about PBL Assay Science?

Visit the manufacturer page at www.stratech.co.uk/pbl_assay_science, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


Product Focus: Z-TECT™ – Streptavidin “Anti-Biotin” MonsterHRP™

The Z-TECT™ HS ELISA Streptavidin MonsterHRP™ Colorimetric Reagent provided is an HRP-conjugated anti-tag secondary reagent that recognizes biotinylated molecules. It is assumed that the user will first build an indirect, sandwich or competitive ELISA on the plate with all plates, buffers and signal reagents provided in the kit.

Streptavidin MonsterHRP™ kit was tested along with most HRP Reagents available, including PolyHRP, and was found to deliver higher detection signal.  See Human IL-22 Detection Kit Comparison below.

Human IL-22 Detection Kit from Competitor E

  • Coating: 4 µg/ml anti-IL22 capture Ab (100 µl/well-Akrivis Coating Buffer), overnight 4°C incubation, 5x Akrivis Wash Buffer
  • Blocking: Akrivis Blocking Buffer (200 µl/well), 1hr@RT incubation, 5x Akrivis Wash Buffer
  • Antigen: IL-22 protein standard (100 µl/well-Akrivis Diluent), 1hr@RT incubation, 5x Akrivis Wash Buffer
  • Primary: 0.25 µg/ml anti-IL22 detection Ab-Biotin (100 µl/well-Akrivis Diluent), 1hr@RT incubation, 5x Akrivis Wash Buffer
  • Secondary: Streptavidin-HRP conjugates (100 µl/well-Akrivis Diluent), 1hr@RT incubation, 5x Akrivis Wash Buffer. All comparators incubated at corresponding manufacturer-recommended concentrations
  • Reading: Akrivis HRP Substrate (100 µl/well), 20min@RT incubation, Akrivis Stop Solution (50 µl/well) and reading at 450 nm

Where can I find more information about US Biological?

Visit the manufacturer page at www.stratech.co.uk/usbio, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.