PBL Assay Science Presentation: Chronic and Acute Markers of Interferon Stimulation

Introduction

  • Interferon (IFN) is implicated in a growing number of conditions such as Lupus, Scleroderma, etc.
  • IFN can be difficult to measure in vivo.
  • Markers allow inference of IFN activity and may offer some discrimination.
  • A panel of IFN and Marker assay(s) for disease differentiation is discussed.

IFN-beta and IFN-gamma differentially up-regulate CXCL-10 (IP-10) in OvCAR cells

Presentation


Where can I find more information about PBL Assay Science?

Visit the manufacturer page at www.stratech.co.uk/pbl_assay_science, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


 

Product Focus: RNA / DNA Preparation and Cleanup – Fast-n-Easy Plasmid-Prep Series

Fast-n-Easy Plasmid-Prep Series is designed for fast and easy isolation of high-purity plasmid or cosmid DNA from bacterial cells for subsequent amplification, sequencing, restriction digests, or transformations. The column based DNA purification follows a simple binding, washing, and eluting procedure. Time-consuming phenolchloroform extraction or alcohol precipitation is not required.

Benefits:

  • yields pure DNA for PCR, sequencing, restriction digest and transformation / transfection
  • saves time
  • come at costs one can’t ignore.

Fast-n-Easy
Plasmid Mini-Prep Kit
Fast-n-Easy
Plasmid Midi-Prep Kit
Fast-n-Easy
Plasmid Maxi-Prep Kit
High DNA Yields up to 20 µg up to 200 µg up to 1 mg
Rapid Purification hands-on time
≤ 10 min
hands-on time
≤ 40 min
hands-on time
≤ 40 min
Reliability pH Indicator for pH
control
color coding of columns for easy handling
Versatility for centrifuge- and vacuum-based purification
Quality high-purity plasmid/cosmid DNA suitable for all downstream application
(e.g. amplification, sequencing, restriction digest and transformation)
Convenience all steps performed at room temperature
no need for phenol-chloroform-extraction or alcohol precipitation
Economy column refill packs available
Stability ≥ 12 months
Fast-n-Easy Plasmid Mini-Prep Kit
Column based isolation of plasmid DNA
Product Cat. No. Amount
Sample PP-204XS 10 preparations
S pack PP-204S 50 preparations
L pack PP-204L 250 preparations
Fast-n-Easy Plasmid Midi-Prep Kit
Column based isolation of plasmid DNA, midi-scale
Product Cat. No. Amount
Sample PP-211XS 4 preparations
20 preparations PP-211S 20 preparations
100 preparations PP-211L 100 preparations
Fast-n-Easy Plasmid Maxi-Prep Kit
Column based isolation of plasmid DNA, maxi-scale
Product Cat. No. Amount
Sample PP-212XS 2 preparations
10 preparations PP-212S 10 preparations
50 preparations PP-212L 50 preparations
Fast-n-Easy Plasmid Mini-Prep Kit – Refill Pack
Binding Columns and Collection Tubes
Product Cat. No. Amount
pack PP-220 100 Binding Columns
Fast-n-Easy Plasmid Midi-Prep Kit – Refill Pack
Pre Columns, Binding Columns and Collection Tubes
Product Cat. No. Amount
pack PP-221 40 Pre Columns
+ 40 Binding Columns
Fast-n-Easy Plasmid Maxi-Prep Kit – Refill Pack
Pre Columns, Binding Columns and Collection Tubes
Product Cat. No. Amount
pack PP-222 20 Pre Columns
+ 20 Binding Columns

Where can I find more information about Jena Bioscience?

Visit the manufacturer page at www.stratech.co.uk/jena_bioscience, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


 

sCD40 Ligand – Canine, Chicken, Equine, & Swine Recombinant Proteins

CD40 Ligand (CD40LG) is a transmembrane protein that is a member of the TNF superfamily. In addition to existing in a membrane bound form, CD40LG can also be released as a soluble molecule (sCD40LG), which has been shown to have biological functions.

CD40 interacts with CD40 ligand (aka CD40LG, TNFSF5 or CD154), which is predominantly expressed on activated CD4+ T-cells. CD40LG is transiently expressed on activated T-cells and plays a crucial role in B-cell function. Activated T-cells expressing CD40LG can also interact with CD40 on endothelial cells to induce production of inflammatory cytokines. The transient expression of CD40LG allows T-cells to stimulate selected CD40-bearing cells to participate in the immune response. Increased levels of sCD40LG have been measured in patients with SLE, RA, inflammatory bowel diseases, systemic sclerosis, and Sjogren’s syndrome. In culture, canine sCD40LG was shown to promote the maturation and activation of canine monocyte derived Dendritic cells.

Kingfisher Biotech now offers canine, chicken, equine, and swine sCD40 Ligand recombinant proteins. The soluble CD40LG recombinant proteins can be used in cell culture, as an CD40LG ELISA Standard, and as a Western Blot Control and are intended for use in animal model and animal health research.

Product
Catalogue No.
Quantity
Canine CD40 Ligand Recombinant Protein – 25 micrograms RP0941D-025 25 ug
Canine CD40 Ligand Recombinant Protein – 5 micrograms RP0941D-005 5 ug
Chicken CD40 Ligand Recombinant Protein – 100 micrograms RP0942C-100 100 ug
Chicken CD40 Ligand Recombinant Protein – 25 micrograms RP0942C-025 25 ug
Chicken CD40 Ligand Recombinant Protein – 5 micrograms RP0942C-005 5 ug
Equine CD40 Ligand Recombinant Protein – 25 micrograms RP0987E-025 25 ug
Equine CD40 Ligand Recombinant Protein – 5 micrograms RP0987E-005 5 ug
Swine CD40 Ligand Recombinant Protein – 25 micrograms RP0936S-025 25 ug
Swine CD40 Ligand Recombinant Protein – 5 micrograms RP0936S-005 5 ug

Where can I find more information about Kingfisher Biotech?

Visit the manufacturer page at www.stratech.co.uk/kingfisher-biotech-inc, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


 

Bovine Leukemia Inhibitory Factor (LIF) Recombinant Protein

Kingfisher Biotech’s Bovine Leukemia Inhibitory Factor (LIF) recombinant protein can be used in cell culture, as an LIF ELISA Standard, and as a Western Blot Control. Leukemia inhibitory factor, or LIF, is a member of the IL-6 family. LIF affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. Leukemia inhibitory factor derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to LIF include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. p53 regulated LIF has been shown to facilitate implantation in the mouse model and possibly in humans. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. LIF is typically added to stem cell culture medium to reduce spontaneous differentiation.Kingfisher Biotech’s Bovine Leukemia Inhibitory Factor (LIF) recombinant protein is intended for use in animal model and animal health research.

Product Catalogue No. Quantity
Bovine Leukemia Inhibitory Factor (LIF) Recombinant Protein – 100 micrograms RP0997B-100 100 ug
Bovine Leukemia Inhibitory Factor (LIF) Recombinant Protein – 25 micrograms RP0997B-025 25 ug
Bovine Leukemia Inhibitory Factor (LIF) Recombinant Protein – 5 micrograms RP0997B-005 5 ug

Where can I find more information about Kingfisher Biotech?

Visit the manufacturer page at www.stratech.co.uk/kingfisher-biotech-inc, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


 

NEW Products: aCella™ – TOX: Bioluminescence Non Radioactive Cytotoxicity Assay (GAPDH)

 Key Benefits

  • Safe – Non radioactive enzyme release assay
  • Versatile – Assay can be run in serum-supplemented media
  • Homogenous – One-step, no wash assay which can be run in same plate as samples
  • FAST – Results in 3-5 minutes
  • Highly Sensitive – can detect less than 500 cells/well in 10% FCS
  • Adaptable for High Throughput screening
  • Non-destructive assay allows monitoring of additional parameters.
  • Perfect for ADCC, CMC & Complement Cytotoxicity assays

Description

Cell Technology offers aCella-TOX, a highly sensitive assay using its patented coupled luminescent technology for the detection of cytotoxicity. This assay is designed to quantitatively measure the release of Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) from mammalian cell lines or bacterial cells (1,2,3,4). Other enzyme release assays suffer from low sensitivity as a result of interference by serum or phenol red present in the media (i.e. Lactate Dehydrogenase (LDH release assay (5,6,7,8)).

The release of GAPDH is coupled with the enzyme 3-Phosphoglyeric Phosphokinase (PGK) to produce ATP. ATP is detected with aCella-TOX as a homogeneous assay that can measure cytoxicity or cell viability via the Luciferase-Luciferin Bioluminescence methodology. It is also possible to set up kinetic assays by transferring culture supernatants from the original assay plate onto a different plate. Additional parameters such as gene expression can also be monitored as the assay system is non-destructive.


Fig A: A Direct comparison of 51Cr and aCella-TOX was carried out with the same donors with Daudi Cells. Log (EC50) value for aCella-Tox was -2.23, and that for 51Cr was -2.087 for Donor A.

Fig B: A Direct comparison of 51Cr and aCella-TOX was carried out with the same donors with Daudi Cells. Log (EC50) value for aCella-TOX was -2.612, and that for 51Cr was -2.77 for Donor B.

Where can I find more information about Cell Technology?

Visit the manufacturer page at www.stratech.co.uk/cell_technology, email info@stratech.co.uk or call  +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


Product Focus: Inhibitors of Angiogenesis Signaling Pathway

Inhibitors of Angiogenesis Signaling Pathway

Inhibitors of Angiogenesis Signaling Pathway

Raf Inhibitors

Cat.No.

Product Name

Information

Product Citations

S1267 Vemurafenib (PLX4032, RG7204) Vemurafenib (PLX4032, RG7204) is a novel and potent inhibitor of B-RafV600E with IC50 of 31 nM.
  • Nature, 2012, 483(7387):100-3
  • Nature, 2014, 508(7494):118-22
  • Cell, 2012, 151(5):937-50
S1040 Sorafenib Tosylate Sorafenib Tosylate (Bay 43-9006) is a multikinase inhibitor of Raf-1, B-Raf and VEGFR-2 with IC50 of 6 nM, 22 nM and 90 nM, respectively.
  • Hepatology, 2013, 59(4):1435-47
  • Blood, 2012, 120(19):4104-15
  • Blood, 2013, 122(9):1621-33
S1152 PLX-4720 PLX4720 is a potent and selective inhibitor of B-RafV600E with IC50 of 13 nM, equally potent to c-Raf-1(Y340D and Y341D mutations), 10-fold selectivity for B-RafV600E than wild-type B-Raf.
  • Nature, 2013, 498(7452):109-12
  • Nature, 2014, 10.1038/nature14136
  • Nature, 2012, 483(7387):100-3
S2807 Dabrafenib (GSK2118436) Dabrafenib (GSK2118436) is a mutant BRAFV600 specific inhibitor with IC50 of 0.8 nM, with 4- and 6-fold less potency against B-Raf(wt) and c-Raf, respectively.
  • J Clin Invest, 2014, 124(3):1406-17
  • J Clin Invest, 2014, 124(11):5074-84
  • Cancer Res, 2014, 10.1158/0008-5472.CAN-14-1392
S1104 GDC-0879 GDC-0879 is a novel, potent, and selective B-Raf inhibitor with IC50 of 0.13 nM with activity against c-Raf as well; no inhibition known to other protein kinases.
  • Cancer Discov, 2013, 3(3):350-62
  • J Natl Cancer Inst, 2012, 104(21):1673-9
  • Proc Natl Acad Sci U S A, 2011, 108(15):6067-72
S7108 Encorafenib (LGX818) Encorafenib (LGX818) is a highly potent RAF inhibitor with selective anti-proliferative and apoptotic activity in cells expressing B-RAF(V600E) with EC50 of 4 nM. Phase 3.
S2161 RAF265 (CHIR-265) RAF265 (CHIR-265) is a potent selective inhibitor of C-Raf/B-Raf/B-Raf V600E with IC50 of 3-60 nM, and exhibits potent inhibition on VEGFR2 phosphorylation with EC50 of 30 nM. Phase 2.
  • Hepatology, 2012, 56(6):2363-74
  • Mol Cell Proteomics, 2012, 11(6):M112.017764
  • Pigment Cell Melanoma Res, 2014, 27(1):124-33
S2746 AZ 628 AZ628 is a new pan-Raf inhibitor for BRAF, BRAFV600E, and c-Raf-1 with IC50 of 105 nM, 34 nM and 29 nM, also inhibits VEGFR2, DDR2, Lyn, Flt1, FMS, etc.
  • Cancer Discov, 2013, 3(3):350-62
  • J Med Chem, 2015, 58(1):466-79
  • Cell Death Dis, 2014, 5:e1278
S2202 NVP-BHG712 NVP-BHG712 is a specific EphB4 inhibitor with ED50 of 25 nM that discriminates between VEGFR and EphB4 inhibition; also shows activity against c-Raf, c-Src and c-Abl with IC50 of 0.395 μM, 1.266 μM and 1.667 μM, respectively.
  • Anticancer Res, 2014, 34(6):2913-8
S2220 SB590885 SB590885 is a potent B-Raf inhibitor with Ki of 0.16 nM, 11-fold greater selectivity for B-Raf over c-Raf, no inhibition to other human kinases.
  • Cell Death Dis, 2014, 5:e1278
  • Invest New Drugs, 2014, 32(4):626-35
S2720 ZM 336372 ZM 336372 is a potent and selective c-Raf inhibitor with IC50 of 70 nM, 10-fold selectivity over B-RAF, no inhibition to PKA/B/C, AMPK, p70S6, etc.
S7397 Sorafenib Sorafenib is a multikinase inhibitor of Raf-1, B-Raf and VEGFR-2 with IC50 of 6 nM, 22 nM and 90 nM, respectively.
  • J Neurosci, 2013, 33(7):3079-93
  • Sci Signal, 2014, 7(322):ra38
  • Mol Oncol, 2014, 8(7):1339-54
S2872 GW5074 GW5074 is a potent and selective c-Raf inhibitor with IC50 of 9 nM, no effect on the activities of JNK1/2/3, MEK1, MKK6/7, CDK1/2, c-Src, p38 MAP, VEGFR2 or c-Fms is noted.
  • Clin Cancer Res, 2014, 20(9):2410-23
  • Mol Cell Proteomics, 2012, 11(9):745-57
S7291 TAK-632 TAK-632 is a potent pan-Raf inhibitor with IC50 of 8.3 nM and 1.4 nM for B-Raf(wt) and C-Raf, respectively, showing less or no inhibition against other tested kinases.
S2200 Raf265 derivative Raf265 derivative, however, the effects of this derivative are not known.
S8015 CEP-32496 CEP-32496 is a highly potent inhibitor of BRAF(V600E/WT) and c-Raf with Kd of 14 nM/36 nM and 39 nM, also potent to Abl-1, c-Kit, Ret, PDGFRβ and VEGFR2, respectively; insignificant affinity for MEK-1, MEK-2, ERK-1 and ERK-2. Phase 1/2.

Where can I find more information about Selleck Chemicals?

Visit the manufacturer page at www.stratech.co.uk/selleck_chemicals, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


 

NEW: hAstroPro Human Astrocyte+Astroglial-Neurons Co-Culturing Kits

Neuromics now provides ArunA Biomedical’s  first of  their kind human astrocyte-glial or astrocyte-glial-neuron co-culturing kits to meet your exacting specifications. Whether you require a pure astro-glial or mixed culture, we are proud to be the first offering you the right solution. Options:

  1. hAstroPro™ Human Astrocyte Cells are derived from human pluripotent stem cell line h9 (NIH registry number 0062) and are the first highly characterized hESC derived CNS support cell type commercially available. hAstroPro™ cells are derived using a chemically defined method that enables shortened differentiation and scaled uniform production.
  2. hAstroPro™+NeuroNet™ Pure Human Neurons are a highly pure population of hPSC derived neurons developed with versatility in mind. As a standalone model for studies of cellular and molecular neurobiology, drug discovery and neurotoxicity, NeuroNet™ Human Neurons enable rapid response in a relevant in vitro culture model system and have been strategically designed for co-planer cellular model studies.

Images: Astrocyte-Glial-Neuron Co-Culture Stained with MAP-2 (Green) and DAPI (Blue).


hAstroPro Human Astrocyte Kit

Cell Type:  Astrocytes
Part Number:  hAP7027
Species:  human
Source:  derived from h9 hESC (NIH registry number 0062)
Morphology:  glial
Quantity:  ~1×106cells per vial
Shipping method:  dry ice
Storage:  liquid nitrogen
Growth Properties:  adherent
Medium:  AB2™ Basal Neural Medium with ANS™ Neural Medium Supplement plus additional unsupplied reagents.
Purity as determinded by qPCR: S100β, CD44, BDNF, GFAP.


NeuroNet Pure Human Neurons

Quantity:  ~1×106 cells per vial
Shipping method:  dry ice
Storage:  liquid nitrogen
Growth Properties:  adherent, non-proliferative
Medium:  AB2™ Basal Neural Medium with ANS™ Neural Medium Supplement plus LIF (10 mg/mL)
Phenotyping:  Cells are >90% β III tubulin positive and >60% MAP2 positive.

DNA fingerprint:  Results are consistent with the presence of a single cell line with no evidence of culture cross contamination. The loci match the DNA fingerprint pattern for the H9 (NIH designation, WA09) hESC line as published in http://stemcells.nih.gov/research/nihresearch/scunit/.

Pathogen testing:   This lot was derived from the H9 hESC line that has been tested for Hepatitis B, Hepatitis C, HIV-1, HIV-2, HTLV-I/II, HSV1, HSV2, EBV, and CMV.  The H9 cell line has been tested and shown to be negative (performed by GIVF Laboratories).  Cells are tested for sterility and mycoplasma contamination.


Where can I find more information about Neuromics?

Visit the manufacturer page at www.stratech.co.uk/neuromics, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe.

Please contact us to find out which product ranges we can supply in your country.


 

Product Focus: Annexin V-FITC Kit

Benefits

  • Detects apoptosis earlier in the process than DNA-based assays (TUNEL).
  • Rapid labeling of cells. Cell staining takes only 10 minutes.
  • No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
  • PI is included with the kit to differentiate apoptotic cells from viable and necrotic cells.

Introduction

Annexin V-FITC kit provides a rapid and convenient assay for apoptosis.Annexin V-FITC kit detects the externalization of phosphatidylserine in apoptotic cells using recombinant annexin V conjugated to green-fluorescent fluorescein isothiocyante (FITC) dye and necrotic cells using propidium iodide (PI) by flow cytometry and fluorescence microscope. The AnnexinV-FITC kit uses annexin V conjugated with FITC to label phosphatidylserine sites on the membrane surface. The kit also includes propidium iodide (PI) stains necrotic cells with red fluorescence. After treatment with both probes, apoptotic cells show green fluorescence, dead cells show red and green fluorescence, and live cells show little or no fluorescence.

Catalogue B32115 (50 rxns) B32117 (100 rxns)
Annexin V-FITC 250 μl 250 μl×2
PI Staining Solution 250 μl 250 μl×2
1 × Binding Buffer 25 ml 25 ml×2

Storage

Store in refrigerator (2–8° C) and protect from light.


Experimental Protocol

1. Induce apoptosis in cells using the desired method. Prepare a negative control by incubating cells in the absence of inducing agent.
2. Harvest the cells after the incubation period and wash in cold phosphate-buffered saline (PBS).
3. Re-centrifuge the washed cells, discard the supernatant, and resuspend the cells in 100 μl 1 × Binding Buffer.
4. Add 5 μL Annexin V -FITC and 5 μL PI Staining Solution to each 100 μL of cell suspension.
5. Incubate the cells at room temperature for 15 minutes.
6. After the incubation period, add 400 μL of 1 × Binding Buffer, mix gently and keep the samples on ice.
7. As soon as possible, analyze the stained cells by flow cytometry, measuring the fluorescence emission at 530 nm (e.g., FL1) and >575 nm (e.g., FL3). The population should separate into three groups: live cells will show only a low level of fluorescence, apoptotic cells show green fluorescence and dead cells show both red and green fluorescence.Confirm the flow cytometry results by viewing the cells under a fluorescence microscope, using filters appropriate for fluorescein (FITC) and rhodamine (TRITC) or Texas RedR dye.

Biotool™ Annexin V-FITC Apoptosis Detection Kit Protocol


Where can I find more information about Biotool?

Visit the manufacturer page at www.stratech.co.uk/biotool, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


 

Product Focus: RNA in vivo transfection reagent

work well for siRNA, miRNA, guide RNA and long ncRNAs


Description

Biotool RNA in vivo Transfection Reagent is a nanoscale polymer based reagent, successfully used to in vivo transfer siRNA, miRNA (mimic and inhibitor), guide RNA or long ncRNAs for research purposes such as gene functional studies or RNAi in vivo studies. This reagent interacts with nucleic acids and moves together into cells via endocytosis. In addition to efficiently transporting nucleic acids into the cells, the formula is also able to lead to the protection of the nucleic acids from degradation in vivo. Biotool RNA in vivo Transfection Reagent has been proven to be able to perform the maximum delivery of siRNA, miRNA (mimic and inhibitor), guide RNA or long ncRNAs into different organs in the body. No significant pro-inflammatory responses have been detected using this reagent.

Biotool RNA in vivo Transfection Reagent performs high transfection efficiency in vivo.

Biotool RNA in vivo Transfection Reagent test: Systemic administration (i.v.) of Biotool RNA in vivo Transfection Reagent conjugated with siRNA (siRNA/mouse: 1mg/kg, 2mg/kg and 3mg/kg) targeting coagulation factor VII (FVII) gene mRNA or non-silencing control siRNA (3mg/kg) following our protocol. Liver was collected and total RNA isolated 48 hours after tail vein injection. Samples were analyzed by qRT-PCR for FVII mRNA expression levels. β-Actin RNA levels were used to normalize the expression data. Data are means +/- SD (n=6).

No detectable inflammatory response.

Serum concentration of IL12/IL23 and IL6 following intravenous nucleic acid delivery using Biotool RNA in vivo Transfection Reagent or saline (negative control) 24h after injection respectively. The positive control is an IP injection of 50μg inactivated E.coli (LPS). 40ug siRNA were delivered with or without Biotool RNA in vivo Transfection Reagent.

Products

B45212     20 injections (0.5 ml)
B45215     100 injections (2.5 ml)
B45218     1000 injections (25 ml)


Flow Chart


 Troubleshooting

 

Problem Possible Reason Suggested Improvement
Precipitate formation in transfection mixture RNA not dissolved in distilled H2O (e.g. Tris-EDTA) Prepare fresh culture.
Excessive RNA concentration in transfection mixture Ensure RNA concentration in transfection mixture ≤ 0.5 µg/µL before injection
Transfection mixture froze or was stored at low temperature for a long period of time Freshly prepare transfection mixture and use immediately
Low transfection efficiency Low administration dose Scale up administration dose in the case of exclusion of animal lethality
Inflammation response or lethality of animal Injection manipulation error Inject the transfection mixture at correct site by correct method
Excessive RNA amount Scale down RNA amount and adjust the volume of transfection reagent accordingly
Endotoxin in transfection mixture Prepare endotoxin-free RNA
Pass transfection mixture through a 0.22 µM sterile filter before injection

 


 User Guide

Biotool RNA in vivo Transfection Reagent.pdf


Where can I find more information about Biotool?

Visit the manufacturer page at www.stratech.co.uk/biotool, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.


 

Product Focus: IDH1 R132H detection: Clone H09 is the gold standard.

In histological studies of human IDH1 R132H in formalin-fixed paraffin-embedded brain tissue sections antibody clone H09 displays a strong and specific reaction at a 1:40 dilution. In contrast, at a 1:40 dilution antibody clone HMab-1 shows a very faint staining which remains weak even at lower dilutions of 1:20 and 1:10.

The exclusive production of antibody clone H09 by dianova together with its widespread commercial availability since 2010 has changed routine neuropathology: It provides an unrevaled accuracy to the identification of infiltrating gliomas and secondary gliomas.

At present many companies non-exclusivly offer a different clone, HMab-1. By comparative IHC analysis we demonstrate that clone H09 displays an unchallenged staining quality at considerably higher dilution.

H09-HMab-FigFig.: IHC of human IDH1 R132H in formalin-fixed paraffin-embedded brain tissue sections: The IDH1 R132H mutation specific monoclonal antibody clone H09 displays a strong and specific reaction at a 1:40 dilution. In contrast, at a 1:40 dilution antibody clone HMab-1 shows a very faint staining which remains weak even at lower dilutions of 1:20 and 1:10. All sections were stained by an indirect alkaline phosphatase method according to standard procedures with antigen retrieval by high-temperature heating in citrate buffer (pH 6.0) and counterstaining with Hematoxylin.


Where can I find more information about Dianova?

Visit the manufacturer page at www.stratech.co.uk/dianova, email info@stratech.co.uk or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.